JCRB1015 HSC-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1015 Cell Name   HSC-1
Profile   A cell line of a squamous cell carcinoma of human skin. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   75
Identity   available Tissue for Primary Cancer   skin
Case history   an ulcerated tumor on the back of the right hand Metastasis   
Tissue Metastasized    Genetics   excess expression of the EGF recepters observed. Modal number of chromosome is reported to be 80.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Skin squamous carcinoma cell line.
Classify   tumor Established by   Kondo,S. and Aso,A.
Registered by   Hozumi,Y. Regulation for Distribution   
Comment   Mouse 3T3 irradiated with X-ray were used as feeder cells when established this cell line. Year   2001
Medium   Dulbecco's modified Eagle's medium with 20 % fetal bovine serum. Methods for Passages   Cells were harvested after treatment with 0.02% EDTA and 0.05% trypsin for three minutes. Subculture cells every 2 weeks.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2807
Reference
Pubmed id:7284243Establishment of a cell line of human skin squamous cell carcinoma in vitro.
Kondo S,Aso K
Br J Dermatol. 1981 Aug;105(2):125-32

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LOT Information

Cell No.   JCRB1015 Cell Name   HSC-1
LOT No.   10082004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 20% fetal bovine serum (FBS lot; GIBCO 3762782S). Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.05% trypsin and 0.02% EDTA
Doubling Time   ca. 1 day Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P93 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   Ampoule mislabeling: JCRB1065 is error, correctly, JCRB1015.
Cell No.   JCRB1015 Cell Name   HSC-1
LOT No.   06242016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   7.0 - 8.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 37 hrs Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   96.8 Antibiotics Used   free
Passage Number   P93 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96.8 Additional information   
Cell No.   JCRB1015 Cell Name   HSC-1
LOT No.   01232025 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (GIBCO:catalog No.11885-084) with 10% fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K). Temperature   37 C
Cell Density at Seeding   1.6-2.2x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   p93 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11,12
D7S820:11,12
D16S539:11,12
VWA:16,17
TH01:7
AM:X,Y
TPOX:8
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
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