JCRB1016 HSC-5

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1016 Cell Name   HSC-5
Profile   A cell line derived from human skin squamous cell carcinoma having anaplastic epithelioid features. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   75
Identity   available Tissue for Primary Cancer   skin
Case history   ulcerated tumor on the upper part of the right auricle. Metastasis   
Tissue Metastasized    Genetics   Modal number of chromosome is reported to be 76.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   The cells lost their ability to defferentiate to keratinize. The cells express cytokeratins 5, 8, 13, 18 and 19 {5064}.
Classify   tumor Established by   Hozumi,Y. et.al.
Registered by   Hozumi,Y. Regulation for Distribution   
Comment    Year   2001
Medium   Iscove's modified Dulbecco's medium containing 10% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.02% EDTA and 0.05% trypsin. Subculture once a week.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0314
Reference
Pubmed id:1693926 Human squamous cell carcinoma from skin: establishment and characterization of a new cell line (HSC-5).
Hozumi Y,Kondo S,Shimoura T,Aso K
J Dermatol. 1990 Mar;17(3):143-8
Pubmed id:6169443 A new small (40 kd) keratin filament protein made by some cultured human squamous cell carcinomas.
Wu YJ,Rheinwald JG
Cell. 1981 Sep;25(3):627-35

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LOT Information

Cell No.   JCRB1016 Cell Name   HSC-5
LOT No.   08062007 Lot Specification   distribution
Medium   Iscove's modified Dulbecco's medium with 10% fetal bovine serum (lot:GIBCO484375). Temperature   37C
Cell Density at Seeding   1.2 x 10^5cells/ml (twice/week) Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   6.0 x 10^6 cells
Viability at cell freezing (%)   85.9% Antibiotics Used   free
Passage Number   P131 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B) AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:9
D7S820:8,11
D16S539:9,12
VWA:16,17
TH01:6,7
AM:X,Y
TPOX:11
CSF1PO:10,11
Adhesion   Yea Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1016 Cell Name   HSC-5
LOT No.   05232018 Lot Specification   distribution
Medium   Iscove's modified Dulbecco's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.8 - 2.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time   approx. 22 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P131 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - IMDM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96.1
Additional information   
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