JCRB1020 RERF-LC-Ad1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1020 | Cell Name | RERF-LC-Ad1 |
|---|---|---|---|
| Profile | Human lung cancer cell line, adenocarcinoma. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 70 |
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | lung cancer, lung adenocarcinoma | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | lung cancer (adeno carcinoma) cell line |
| Classify | tumor | Established by | Teraoka,S. & Kyoizumi,S. et.al. |
| Registered by | Kyoizumi,S. | Regulation for Distribution | not defined |
| Comment | Year | 2001 | |
| Medium | RPMI1640 medium with 10% heat inactivated fetal calf serum. | Methods for Passages | Cells harvested after treatment with 0.05% trypsin and 0.02% EDTA at 37 C for 5 minutes. Subculture once in two weeks. |
| Cell Number on Passage | split ratio = 1/4 | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1020 | Cell Name | |
|---|---|---|---|
| LOT No. | 04132008 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS Lot; GIBCO Cat. No. 10099, Lot 484375) | Temperature | 37 C |
| Cell Density at Seeding | 1.0 - 2.5 x 10^5 cells/ml | Methods for Passages | 0.05% trypsin and 0.02% EDTA at 37 C for 5 min. |
| Doubling Time | approx. 65 hrs | Cell Number in Vial (cells/1ml) | 7.6 x 10^6 |
| Viability at cell freezing (%) | 90.7 | Antibiotics Used | free |
| Passage Number | P22 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1020 | Cell Name | |
|---|---|---|---|
| LOT No. | 09052002 | Lot Specification | seed/1st distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | 0.25% trypsin and 0.02% EDTA | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4.7 x 10^5 | |
| Viability at cell freezing (%) | 74.5 | Antibiotics Used | free |
| Passage Number | P17 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:13 D7S820:11,12 D16S539:9,12 VWA:14,17 TH01:6,9 AM:X TPOX:8,11 CSF1PO:12,13 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1020 | Cell Name | RERF-LC-Ad1 |
|---|---|---|---|
| LOT No. | 07072015 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.41-2.72x10^4cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA at 37 C for 5 minutes |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.62x10^6 |
| Viability at cell freezing (%) | 92 | Antibiotics Used | free |
| Passage Number | p23 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:13 D7S820:11,12 D16S539:9,12 VWA:14,17 TH01:6,9 AM:X TPOX:8,11 CSF1PO:12,13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1020 | Cell Name | RERF-LC-Ad1 |
|---|---|---|---|
| LOT No. | 06192020 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 0.5 - 1.7 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 64 hrs. | Cell Number in Vial (cells/1ml) | 3.1 x 10^6 |
| Viability at cell freezing (%) | 99.0 | Antibiotics Used | free |
| Passage Number | P26 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 87.7 | Additional information |