JCRB1025 MKN7

Cell information

Important Notice(s)

Comment to the description of MKN7 cell line in "Human Cell Culture, Vol II"

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1025 Cell Name   MKN7
Profile   tubular adenocarcinoma, well differentiated Other Name   MKN7
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   stomach
Case history   stomach cancer Metastasis   
Tissue Metastasized    Genetics   stomach, well differentiated tubular adenocarcinoma
Life Span   infinite Crisis PDL   
Morphology    Character   stomach,well differentiated tubular adenocarcinoma
Classify   tumor Established by   Hojo,H.
Registered by   Motoyama & Watanabe Regulation for Distribution   
Comment   39 year-old, male. Mycoplasma-eliminated cells by MC-210. Year   1989
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/5 Race   
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1417
Reference
Pubmed id:2736210Production of transforming growth factor-alpha in human tumour cell lines.
Imanishi K,Yamaguchi K,Suzuki M,Honda S,Yanaihara N,Abe K
Br J Cancer. 1989 May;59(5):761-5
Pubmed id:3095279Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel.
Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M
Jpn J Cancer Res. 1986 Sep;77(9):849-53
Pubmed id:3962675Comparison of seven cell lines derived from human gastric carcinomas.
Motoyama T,Hojo H,Watanabe H
Acta Pathol Jpn. 1986 Jan;36(1):65-83

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LOT Information

Cell No.   JCRB1025 Cell Name   
LOT No.   08242012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3.3 - 4.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 53 hrs Cell Number in Vial (cells/1ml)   9.7 x 10^5 cells/mL
Viability at cell freezing (%)   92.9 Antibiotics Used   free
Passage Number   Unknown (>30) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1025 Cell Name   
LOT No.   02012011 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.2 - 2.7 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   57 - 87 hrs. Cell Number in Vial (cells/1ml)   7.4 x 10^5
Viability at cell freezing (%)   81.3 Antibiotics Used   free
Passage Number   Unknown (29 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1025 Cell Name   
LOT No.   06252009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   50 - 70 hrs Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   73.4 Antibiotics Used   free
Passage Number   Unknown (>29) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1025 Cell Name   
LOT No.   07062007 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   2-3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   5.8 x 10^5
Viability at cell freezing (%)   81.5 Antibiotics Used   free
Passage Number   Unknown ( >P34) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1025 Cell Name   
LOT No.   03242003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml. Methods for Passages   0.25% trypsin and 0.02% EDTA for 10-15 min at 37 C
Doubling Time    Cell Number in Vial (cells/1ml)   1.0 X 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   >P31 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS in RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1025 Cell Name   MKN7
LOT No.   08092018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.9 - 4.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 53 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   92.6 Antibiotics Used   free
Passage Number   Unknown (28 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   88.8 Additional information   
Cell No.   JCRB1025 Cell Name   MKN7
LOT No.   08192020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% ftal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3.7 - 7.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA for 10 min at 37 C.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   94.9 Antibiotics Used   free
Passage Number   Unknown (29 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   97.4 Additional information   
Cell No.   JCRB1025 Cell Name   MKN7
LOT No.   06052023 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum(SIGMA,　Cat#172012,　Lot#12J396) Temperature   37 C
Cell Density at Seeding   0.444-2.18x10^4 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin (GIBCO) and 0.02% EDTA for 10 minutes at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.52x10^6
Viability at cell freezing (%)   99.4 Antibiotics Used   free
Passage Number   p30* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,13
D13S317:8,12
D7S820:9
D16S539:9,10
VWA:17
TH01:7,9
AM:X
TPOX:9
CSF1PO:10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   87.8 Additional information   
Images
Cell No.   JCRB1025 Cell Name   MKN7
LOT No.   04112023 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   37 C
Cell Density at Seeding   1.0 - 4.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   6.0 x 10^5
Viability at cell freezing (%)   99.2 Antibiotics Used   free
Passage Number   Unknown (>29) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,13
D13S317:8,12
D7S820:9
D16S539:9,10
VWA:17
TH01:7,9
AM:X
TPOX:9
CSF1PO:10,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   99.2 Additional information   
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