JCRB1026 OV3121

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1026 Cell Name   OV3121
Profile   A non-metastatic epitherial tumor cell line established from ovarian granulosa cell tumor irradiated with 60-Co-gamma rays. Cells are producing estradiol continuously. Other Name   
Animal   mouse Strain   B6C3F1
Genus   Mus Species   musclus
Sex   F Age   
Identity    Tissue for Primary Cancer   ovary
Case history   60-Co-irradiated. Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Non-metastatic epithelial tumor cells from granulosa cell of mouse irradiated with 60-Co-gamma rays. Cells are producing estradiol and angiogenesis was observed when transplanted to mice. Cells grow in monolayer with 28-30 hrs' population doubling.
Classify   tumor Established by   Yanagihara,K. et.al.
Registered by   Yanagihara,K. Regulation for Distribution   
Comment    Year   2001
Medium   RPMI1640 medium with heat-inactivated 10% fetal calf serum or Dulbecco's modified Eagle's medium with heat-inactivated 10% fetal calf serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   split ratio = 1/10. Race   
CO2 Conc.   5 % Tissue Sampling   ovarian granulosa
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_6547
Reference
Pubmed id:7775256A radiation-induced murine ovarian granulosa cell tumor line: introduction of v-ras gene potentiates a high metastatic ability.
Yanagihara K,Nii M,Tsumuraya M,Numoto M,Seito T,Seyama T
Jpn J Cancer Res. 1995 Apr;86(4):347-56

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LOT Information

Cell No.   JCRB1026 Cell Name   
LOT No.   04142009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   5 - 10 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.4 day Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   84.9 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1026 Cell Name   OV3121
LOT No.   12032002 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin. Subculture once a week. Medium change once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   88.6 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP, LDH examined. Mouse.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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