JCRB1027 SAT

Cell information

Important Notice(s)

JCRB1027 SAT細胞株における微量のマウス細胞の混在について

Intermingling of very small amount of mouse cells in JCRB1027 SAT cell line

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1027 Cell Name   SAT
Profile   Human squamous cell carcinoma with non-metastatic cell line derived from oral cavity. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   oral
Case history   squamous cell carcinoma of oral cavity. Metastasis   
Tissue Metastasized    Genetics   MMP and TIMP-1 were examined by the originator {5086}.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells grow in monolayer with doubling time of 72 hrs. Non-metastatic characteristics.
Classify   tumor Established by   Yanagihara,K.
Registered by   Yanagihara,K. Regulation for Distribution   not defined
Comment    Year   2001
Medium   RPMI1640 medium with 10% fetal calf serum or Dulbecco's modified Eagle's medium with 10% fetal calf serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   split ratio = 1/10 - 1/20. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   oral cavity
Tissue Type   squamous cell carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3160
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1299050
Images
Movies



LOT Information

Cell No.   JCRB1027 Cell Name   
LOT No.   06042008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1027 Cell Name   SAT
LOT No.   02032003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   2.1 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Subculture once a week and medium change once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   98.8 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:10,12
D7S820:11,12
D16S539:10,12
VWA:14,18
TH01:9
AM:X
TPOX:9,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1027 Cell Name   SAT
LOT No.   09062016 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   6.3 - 6.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 44 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
CLOSE