JCRB1028 JHH-2

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On the agreement for distribution of JHH series cell lines, NOZ and OZ

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1028 Cell Name   JHH-2
Profile   Human hepatocellular carcinoma cell line (Ed.II). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   57
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   hepatocellular carcinoma. HBs-Ag(-), HBs-Ab(-), AFP=6,100ng/ml, CEA 4.6 ng/ml. Metastasis   Yes
Tissue Metastasized   ascites Genetics   Integration of the HBV-DNA was not observed.
Life Span   infinite Crisis PDL   
Morphology   spindle shaped cells Character   hepatoma (Ed.II). HBs-Ag(-). The cells can be transplantable to nude mice. Mode of chromosome is 70.
Classify   tumor Established by   S.Honma & Nagamori,S.
Registered by   Nagamori,S. Regulation for Distribution   Require negotiation and agreement from Dr.Nagamori before shipping.
Comment   Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. Year   1988
Medium   Williams'E medium with 10% fetal calf serum originally and later 2% FCS is used for 5 years. (alternative MEM 90% + FBS 10%) Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1 x 10^5 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2786
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240157
Reference
Pubmed id:1701409 Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:2484807 [Protein secretion of human cultured liver cells].
Nagamori S,Fujise K,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Hum Cell. 1988 Dec;1(4):382-90


Research results by users. Click!
Pubmed id:22309595 Fibroblast growth factor 19 expression correlates with tumor progression and poorer prognosis of hepatocellular carcinoma.
Miura S,Mitsuhashi N,Shimizu H,Kimura F,Yoshidome H,Otsuka M,Kato A,Shida T,Okamura D,Miyazaki M
BMC Cancer. 2012 Feb 6;12():56
Pubmed id:20044606 Infrequent amplification of JUN in hepatocellular carcinoma.
Endo M,Yasui K,Nakajima T,Gen Y,Tsuji K,Dohi O,Zen K,Mitsuyoshi H,Minami M,Itoh Y,Taniwaki M,Tanaka S,Arii S,Okanoue T,Yoshikawa T
Anticancer Res. 2009 Dec;29(12):4989-94
Pubmed id:17295779 Cimetidine inhibits epidermal growth factor-induced cell signaling.
Fujikawa T,Shiraha H,Nakanishi Y,Takaoka N,Ueda N,Suzuki M,Shiratori Y
J Gastroenterol Hepatol. 2007 Mar;22(3):436-43
Pubmed id:9446792 Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1.
Tsuji T,Miyazaki M,Fushimi K,Mihara K,Inoue Y,Ohashi R,Ohtsubo M,Hamazaki K,Furusako S,Namba M
Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21
Pubmed id:8835345 Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
Tsuboi S,Nagamori S,Miyazaki M,Mihara K,Fukaya K,Teruya K,Kosaka T,Tsuji T,Namba M
J Med Virol. 1996 Feb;48(2):133-40
Pubmed id:1315167 Induction of allogeneic tumour- and lymphokine-activated lymphocytes against hepatocellular carcinoma.
Yasumura S,Higuchi K,Hioki O,Okada K,Tsukishiro T,Tsuchida T,Miyagiwa M,Nambu S,Yasuyama T,Inoue K
J Gastroenterol Hepatol. 1992 Mar-Apr;7(2):136-41
Pubmed id:1701409 Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:3005723 [Effects of hyperthermia on the proliferation and viability of cultured human hepatobiliary carcinoma cells].
Homma S,Nagamori S,Fujise K,Sujino H,Matsuura T,Simizu K,Kameda H
Nihon Shokakibyo Gakkai Zasshi. 1985 Sep;82(9):2043-8
Pubmed id:none THE MORPHOLOGICAL STUDY OF HUMAN CULTURED HEPATOMA CELLS BY A NEW PLASMA POLYMERIZATION REPLICA METHOD AND TEM
Hajime SUJINO
Jikeikai Medical Journal 33(4), 379-400 (1986)
Pubmed id:none 樹立ヒト肝癌株細胞中のB型肝炎ウイルスゲノム
藤瀬 清隆, 藤多 和信, 永森 静志, 蓮村 哲, 本間 定, 筋野 甫, 松浦 知和, 清水 恵一郎, 新谷 稔, 大野 典也, 亀田 治男
肝臓 29(5), 697-698 (1988)

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LOT Information

Cell No.   JCRB1028 Cell Name   
LOT No.   09072011 Lot Specification   distribution
Medium   Williams' medium E with 2% fetal bovine serum (FBS: GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.0 - 1.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 33 hrs. Cell Number in Vial (cells/1ml)   8.2 x 10^5
Viability at cell freezing (%)   93.1 Antibiotics Used   free
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams' medium E. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1028 Cell Name   
LOT No.   11022010 Lot Specification   distribution
Medium   Williams' medium E with 2% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days. Cell Number in Vial (cells/1ml)   5.1 x 10^5
Viability at cell freezing (%)   83.6 Antibiotics Used   fre
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams' medium E CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1028 Cell Name   
LOT No.   01222008 Lot Specification   distribution
Medium   Williams' E medium with 2% FBS. A higher serum concentration (10%) can be used for good growth. (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   35 - 70 hrs Cell Number in Vial (cells/1ml)   6.0 x 10^5
Viability at cell freezing (%)   94.4 Antibiotics Used   free
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams' E medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1028 Cell Name   JHH-2
LOT No.   02072003 Lot Specification   distribution
Medium   Williams' E medium with 2% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   4.0 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.2% trypsin and 0.02% EDTA. Subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   75.6 Antibiotics Used   free
Passage Number   P22 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1028 Cell Name   JHH-2
LOT No.   05022018 Lot Specification   distribution
Medium   Williams' medium E with 2% fetal bovine serum (FBS: Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.3 - 3.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 59 hrs. Cell Number in Vial (cells/1ml)   9.3 x 10^5
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams' medium E CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90.0
Additional information   
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