JCRB1029 JHH-5

Cell information

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On the agreement for distribution of JHH series cell lines, NOZ and OZ

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1029 Cell Name   JHH-5
Profile   Human hepatocellular carcinoma (Ed.I) established from small liver cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   50
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   small liver cancer observed in the chronic hepatitis Metastasis   No
Tissue Metastasized    Genetics   Integration of the HBV-DNA was not observed.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Hepatocellular carcinoma Ed.I. Human albumin secretion and AFP(110ng/ml/48hrs) observed in the supernatant of the cell culture. HBs-Ag(-).
Classify   tumor Established by   Sujino,H., Matsuura,T.&Nagamori,S.
Registered by   Nagamori,S. Regulation for Distribution   Require negotiation and agreement from Dr.Nagamori before shipping.
Comment   Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. Year   2001
Medium   Williams'E medium with 10% fetal calf serum. Methods for Passages   Cells are harvested with 0.2% trypsin and 0.02% EDTA.
Cell Number on Passage   not defined Race   Japanese
CO2 Conc.   5 % Tissue Sampling   resected liver sample
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0364
Reference
Pubmed id:1701409Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:2484807[Protein secretion of human cultured liver cells].
Nagamori S,Fujise K,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Hum Cell. 1988 Dec;1(4):382-90


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Pubmed id:22309595 Fibroblast growth factor 19 expression correlates with tumor progression and poorer prognosis of hepatocellular carcinoma.
Miura S,Mitsuhashi N,Shimizu H,Kimura F,Yoshidome H,Otsuka M,Kato A,Shida T,Okamura D,Miyazaki M
BMC Cancer. 2012 Feb 6;12():56
Pubmed id:20044606 Infrequent amplification of JUN in hepatocellular carcinoma.
Endo M,Yasui K,Nakajima T,Gen Y,Tsuji K,Dohi O,Zen K,Mitsuyoshi H,Minami M,Itoh Y,Taniwaki M,Tanaka S,Arii S,Okanoue T,Yoshikawa T
Anticancer Res. 2009 Dec;29(12):4989-94
Pubmed id:15349899 Proteomic signature corresponding to alpha fetoprotein expression in liver cancer cells.
Yokoo H,Kondo T,Fujii K,Yamada T,Todo S,Hirohashi S
Hepatology. 2004 Sep;40(3):609-17
Pubmed id:15205326 Involvement of interferon regulatory factor 1 and S100C/A11 in growth inhibition by transforming growth factor beta 1 in human hepatocellular carcinoma cells.
Miyazaki M,Sakaguchi M,Akiyama I,Sakaguchi Y,Nagamori S,Huh NH
Cancer Res. 2004 Jun 15;64(12):4155-61
Pubmed id:10766424 Involvement of insulin-like growth factor binding protein-3 in the retinoic acid receptor-alpha-mediated inhibition of hepatocellular carcinoma cell proliferation.
Murakami K,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I
Cancer Lett. 2000 Apr 3;151(1):63-70
Pubmed id:10933057 Invasiveness of hepatocellular carcinoma cell lines: contribution of membrane-type 1 matrix metalloproteinase.
Murakami K,Sakukawa R,Ikeda T,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I
Neoplasia. 1999 Nov;1(5):424-30
Pubmed id:9446792 Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1.
Tsuji T,Miyazaki M,Fushimi K,Mihara K,Inoue Y,Ohashi R,Ohtsubo M,Hamazaki K,Furusako S,Namba M
Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21
Pubmed id:8835345 Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
Tsuboi S,Nagamori S,Miyazaki M,Mihara K,Fukaya K,Teruya K,Kosaka T,Tsuji T,Namba M
J Med Virol. 1996 Feb;48(2):133-40
Pubmed id:1701409 Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:2856500 [Effects of TNF on human hepatocellular carcinoma cell lines and their modification by hyperthermia].
Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Satomi N
Hum Cell. 1988 Jun;1(2):238-44
Pubmed id:none ヒト肝癌細胞株に対するTumor necrosis factorの影響
蓮村哲 永森静志 藤瀬清隆 本間定 筋野甫 松浦知和 清水恵一郎 新谷稔 里見信子
医学のあゆみ 143(8), 655-656 (1987)
Pubmed id:none 樹立ヒト肝癌株細胞中のB型肝炎ウイルスゲノム
藤瀬 清隆, 藤多 和信, 永森 静志, 蓮村 哲, 本間 定, 筋野 甫, 松浦 知和, 清水 恵一郎, 新谷 稔, 大野 典也, 亀田 治男
肝臓 29(5), 697-698 (1988)
Pubmed id:none 株化肝細胞癌の遺伝子発現　Gene expression in human hepatocellular carcinoma cell lines
村上 孝司 山田 雄次 済木 育夫 松浦 知和 蓮村 哲 永森 静志
組織培養研究 18(3), 221-228 (1999)

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LOT Information

Cell No.   JCRB1029 Cell Name   
LOT No.   03022011 Lot Specification   distribution
Medium   Williams' E medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1.2 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   94.9 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams' E medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1029 Cell Name   JHH-5
LOT No.   07152009 Lot Specification   distribution
Medium   Williams'E medium with10% heat inactivated fetal bovine serum(Hyclone GRD0051). Temperature   37 C
Cell Density at Seeding   1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%(GIBCO) trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   96.8 Antibiotics Used   NT
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:9
D7S820:10,12
D16S539:9,10
VWA:17,19
TH01:9
AM:X,Y
TPOX:11
CSF1PO:9
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1029 Cell Name   JHH-5
LOT No.   03262003 Lot Specification   distribution
Medium   William's E medium containing 10% fetal calf serum(Intergen RB52305). Temperature   37 C
Cell Density at Seeding   4.2 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.20% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human, not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:9
D7S820:10,12
D16S539:9,10
VWA:17,19
TH01:9
AM:XY
TPOX:11
CSF1PO:9
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1029 Cell Name   
LOT No.   03112003 Lot Specification   distribution
Medium   William's E medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/sq.cm Methods for Passages   0.2% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 40 hrs Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   91.7 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1029 Cell Name   JHH-5
LOT No.   10242014 Lot Specification   distribution
Medium   William's E medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:9
D7S820:10,12
D16S539:9,10
VWA:17,19
TH01:9
AM:X,Y
TPOX:11
CSF1PO:9
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams E medium CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
Cell No.   JCRB1029 Cell Name   JHH-5
LOT No.   09272018 Lot Specification   distribution
Medium   Williams' E medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.6 - 2.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   93.4 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams' E medium CO2 Conc.   5%
Viability immediately after thawing (%)   91.6 Additional information   
Cell No.   JCRB1029 Cell Name   JHH-5
LOT No.   08252021 Lot Specification   distribution
Medium   Williams' E medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   0.9 - 1.6 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - William's E medium CO2 Conc.   5%
Viability immediately after thawing (%)   94.1 Additional information   
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