JCRB1030 JHH-6

Cell information

Important Notice(s)

On the agreement for distribution of JHH series cell lines, NOZ and OZ

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1030 Cell Name   JHH-6
Profile   Human hepatocellular carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   57
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   anhepatic liver carcinoma, hetocellular carcinoma (Ed.II). HBs-Ag(-), HBs-Ab(-), HBe-Ag(-), HBc-Ab(-), AFP(ND), CEA 4.6ng/ml. Metastasis   
Tissue Metastasized    Genetics   HBV-DNA was not integrated
Life Span   infinite Crisis PDL   
Morphology   ephitherial-like structure Character   Undifferentiated morphology observed. Albumin, AFP and CEA were not observed in the supernatant of the cell culture but ferritin was observed (6.7 ng/ml). HBs-Ag(-), HBs-Ag(-).
Classify   tumor Established by   Matsuura,T. & Nagamori,S.
Registered by   Nagamori,S. Regulation for Distribution   Require negotiation and agreement from Dr.Nagamori before shipping.
Comment   Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. Year   2001
Medium   Williams'E medium with 10% fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   not defined Race   Japanese
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   undifferentiated hepato cellular carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2788
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240159
Reference
Pubmed id:1701409 Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:2484807 [Protein secretion of human cultured liver cells].
Nagamori S,Fujise K,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Hum Cell. 1988 Dec;1(4):382-90


Research results by users. Click!
Pubmed id:24574752 Bortezomib effect on E2F and cyclin family members in human hepatocellular carcinoma cell lines.
Baiz D,Dapas B,Farra R,Scaggiante B,Pozzato G,Zanconati F,Fiotti N,Consoloni L,Chiaretti S,Grassi G
World J Gastroenterol. 2014 Jan 21;20(3):795-803
Pubmed id:23967134 Specific inhibition of the redox activity of ape1/ref-1 by e3330 blocks tnf-α-induced activation of IL-8 production in liver cancer cell lines.
Cesaratto L,Codarin E,Vascotto C,Leonardi A,Kelley MR,Tiribelli C,Tell G
PLoS One. 2013;8(8):e70909
Pubmed id:24116172 The expression of CD90/Thy-1 in hepatocellular carcinoma: an in vivo and in vitro study.
Sukowati CH,Anfuso B,Torre G,Francalanci P,Crocè LS,Tiribelli C
PLoS One. 2013;8(10):e76830
Pubmed id:22309595 Fibroblast growth factor 19 expression correlates with tumor progression and poorer prognosis of hepatocellular carcinoma.
Miura S,Mitsuhashi N,Shimizu H,Kimura F,Yoshidome H,Otsuka M,Kato A,Shida T,Okamura D,Miyazaki M
BMC Cancer. 2012 Feb 6;12():56
Pubmed id:21882038 Mitochondrial bioenergetic profile and responses to metabolic inhibition in human hepatocarcinoma cell lines with distinct differentiation characteristics.
Domenis R,Comelli M,Bisetto E,Mavelli I
J Bioenerg Biomembr. 2011 Oct;43(5):493-505
Pubmed id:21831731 Effects of E2F1-cyclin E1-E2 circuit down regulation in hepatocellular carcinoma cells.
Farra R,Dapas B,Pozzato G,Scaggiante B,Agostini F,Zennaro C,Grassi M,Rosso N,Giansante C,Fiotti N,Grassi G
Dig Liver Dis. 2011 Dec;43(12):1006-14
Pubmed id:20144681 Serum response factor depletion affects the proliferation of the hepatocellular carcinoma cells HepG2 and JHH6.
Farra R,Dapas B,Pozzato G,Giansante C,Heidenreich O,Uxa L,Zennaro C,Guarnieri G,Grassi G
Biochimie. 2010 May;92(5):455-63
Pubmed id:20200618 Galectin-1 and its involvement in hepatocellular carcinoma aggressiveness.
Spano D,Russo R,Di Maso V,Rosso N,Terracciano LM,Roncalli M,Tornillo L,Capasso M,Tiribelli C,Iolascon A
Mol Med. 2010 Mar;16(3-4):102-15
Pubmed id:20044606 Infrequent amplification of JUN in hepatocellular carcinoma.
Endo M,Yasui K,Nakajima T,Gen Y,Tsuji K,Dohi O,Zen K,Mitsuyoshi H,Minami M,Itoh Y,Taniwaki M,Tanaka S,Arii S,Okanoue T,Yoshikawa T
Anticancer Res. 2009 Dec;29(12):4989-94
Pubmed id:19041685 Bortezomib arrests the proliferation of hepatocellular carcinoma cells HepG2 and JHH6 by differentially affecting E2F1, p21 and p27 levels.
Baiz D,Pozzato G,Dapas B,Farra R,Scaggiante B,Grassi M,Uxa L,Giansante C,Zennaro C,Guarnieri G,Grassi G
Biochimie. 2009 Mar;91(3):373-82
Pubmed id:19142034 Contribution of AP-1 interference induced by TAC-101 to tumor growth suppression in a hepatocellular carcinoma model.
Eshima K,Fukaya S,Sugimoto A,Mori T,Yokoi H,Yamamoto Y,Sugiura S,Honda S,Masuko N,Murakami K,Yamasaki Y,Kagechika H
Tumour Biol. 2009;30(1):1-7
Pubmed id:17825975 The expression levels of the translational factors eEF1A 1/2 correlate with cell growth but not apoptosis in hepatocellular carcinoma cell lines with different differentiation grade.
Grassi G,Scaggiante B,Farra R,Dapas B,Agostini F,Baiz D,Rosso N,Tiribelli C
Biochimie. 2007 Dec;89(12):1544-52
Pubmed id:15349899 Proteomic signature corresponding to alpha fetoprotein expression in liver cancer cells.
Yokoo H,Kondo T,Fujii K,Yamada T,Todo S,Hirohashi S
Hepatology. 2004 Sep;40(3):609-17
Pubmed id:10766424 Involvement of insulin-like growth factor binding protein-3 in the retinoic acid receptor-alpha-mediated inhibition of hepatocellular carcinoma cell proliferation.
Murakami K,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I
Cancer Lett. 2000 Apr 3;151(1):63-70
Pubmed id:10933057 Invasiveness of hepatocellular carcinoma cell lines: contribution of membrane-type 1 matrix metalloproteinase.
Murakami K,Sakukawa R,Ikeda T,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I
Neoplasia. 1999 Nov;1(5):424-30
Pubmed id:9446792 Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1.
Tsuji T,Miyazaki M,Fushimi K,Mihara K,Inoue Y,Ohashi R,Ohtsubo M,Hamazaki K,Furusako S,Namba M
Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21
Pubmed id:8835345 Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
Tsuboi S,Nagamori S,Miyazaki M,Mihara K,Fukaya K,Teruya K,Kosaka T,Tsuji T,Namba M
J Med Virol. 1996 Feb;48(2):133-40
Pubmed id:1701409 Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:none 樹立ヒト肝癌株細胞中のB型肝炎ウイルスゲノム
藤瀬 清隆, 藤多 和信, 永森 静志, 蓮村 哲, 本間 定, 筋野 甫, 松浦 知和, 清水 恵一郎, 新谷 稔, 大野 典也, 亀田 治男
肝臓 29(5), 697-698 (1988)
Pubmed id:none 株化肝細胞癌の遺伝子発現 Gene expression in human hepatocellular carcinoma cell lines
村上 孝司 山田 雄次 済木 育夫 松浦 知和 蓮村 哲 永森 静志
組織培養研究 18(3), 221-228 (1999)

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LOT Information

Cell No.   JCRB1030 Cell Name   JHH-6
LOT No.   09082009 Lot Specification   distribution
Medium   Williamms' E medium with 10% fetal bovine serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.6x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%(GIBCO) trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   98.8 Antibiotics Used   NT
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:11,12,13
D7S820:11
D16S539:10
VWA:14,18
TH01:7,9
AM:X
TPOX:8,9
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1030 Cell Name   JHH-6
LOT No.   04182003 Lot Specification   distribution
Medium   William's E medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.2% trypsin. Medium change twice a week. Subculture every 4-5 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   98.3 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   culture medium containing 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1030 Cell Name   
LOT No.   03282003 Lot Specification   distribution
Medium   William's E medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   2-3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDAT
Doubling Time   D.T. = ca. 30 hrs Cell Number in Vial (cells/1ml)   9.6 x 10^5
Viability at cell freezing (%)   86.4 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS-culture CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1030 Cell Name   JHH-6
LOT No.   05182017 Lot Specification   distribution
Medium   Williams'E medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.2.6 - 9.3 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   95.9 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams'E medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95.9
Additional information   
Cell No.   JCRB1030 Cell Name   JHH-6
LOT No.   06212018 Lot Specification   distribution
Medium   Williams'E medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.0 - 3.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   8.7 x 10^5
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams'E medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95.5
Additional information   
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