JCRB1031 JHH-7
Cell information
Important Notice(s)JCRB1031 JHH-7: HBV Integration
On the agreement for distribution of JHH series cell lines, NOZ and OZ
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1031 | Cell Name | JHH-7 |
|---|---|---|---|
| Profile | HBV integrated human hepato cellular carcinoma cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 53 |
| Identity | available | Tissue for Primary Cancer | liver, gallbladder |
| Case history | HBs-Ag positive hepato cellular carcinoma (Ed.III) with liver cirrhosis. HBs-Ag(+), HBs-Ab(-), HBe-Ag(-), HBe-Ab(+), HBc-Ab(+), AFP positive (110,000 ng/ml). | Metastasis | |
| Tissue Metastasized | Genetics | HBV-DNA integration observed. 6.0/2.5Kb bands by HindIII were observed. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | |
| Classify | tumor | Established by | Honma,S. Fujise,K. Nagamori,S. |
| Registered by | Nagamori,S. | Regulation for Distribution | Require negotiation and agreement from Dr.Nagamori before shipping. |
| Comment | Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. | Year | 2001 |
| Medium | William's E medium with 10% fetal calf serum. | Methods for Passages | Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Cell Number on Passage | Not defined. | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | liver |
| Tissue Type | hepato cellular carcinoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | + | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | PCR test for HB virus genome fragment was positive, but the integrated HB virus genome was incomplete, and therefore HB virus is not produced by JHH-7. Nihon Shokakibyo Gakkai Zasshi. 1990 Sep;87(9):1851-5. | ||||||||
| Reference | |
|---|---|
| Pubmed id:1701409 | Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines. Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K Hepatogastroenterology. 1990 Oct;37(5):457-60 |
| Pubmed id:2484807 | [Protein secretion of human cultured liver cells]. Nagamori S,Fujise K,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H Hum Cell. 1988 Dec;1(4):382-90 |
| Research results by users. Click！ | |
|---|---|
| Pubmed id:22309595 |
Fibroblast growth factor 19 expression correlates with tumor progression and poorer prognosis of hepatocellular carcinoma. Miura S,Mitsuhashi N,Shimizu H,Kimura F,Yoshidome H,Otsuka M,Kato A,Shida T,Okamura D,Miyazaki M BMC Cancer. 2012 Feb 6;12():56 |
| Pubmed id:21725613 |
Growth inhibition by NVP-BEZ235, a dual PI3K/mTOR inhibitor, in hepatocellular carcinoma cell lines. Masuda M,Shimomura M,Kobayashi K,Kojima S,Nakatsura T Oncol Rep. 2011 Nov;26(5):1273-9 |
| Pubmed id:20044606 |
Infrequent amplification of JUN in hepatocellular carcinoma. Endo M,Yasui K,Nakajima T,Gen Y,Tsuji K,Dohi O,Zen K,Mitsuyoshi H,Minami M,Itoh Y,Taniwaki M,Tanaka S,Arii S,Okanoue T,Yoshikawa T Anticancer Res. 2009 Dec;29(12):4989-94 |
| Pubmed id:19142034 |
Contribution of AP-1 interference induced by TAC-101 to tumor growth suppression in a hepatocellular carcinoma model. Eshima K,Fukaya S,Sugimoto A,Mori T,Yokoi H,Yamamoto Y,Sugiura S,Honda S,Masuko N,Murakami K,Yamasaki Y,Kagechika H Tumour Biol. 2009;30(1):1-7 |
| Pubmed id:16160184 |
Formation of vesicular stomatitis virus pseudotypes bearing surface proteins of hepatitis B virus. Saha MN,Tanaka A,Jinno-Oue A,Shimizu N,Tamura K,Shinagawa M,Chiba J,Hoshino H J Virol. 2005 Oct;79(19):12566-74 |
| Pubmed id:15349899 |
Proteomic signature corresponding to alpha fetoprotein expression in liver cancer cells. Yokoo H,Kondo T,Fujii K,Yamada T,Todo S,Hirohashi S Hepatology. 2004 Sep;40(3):609-17 |
| Pubmed id:12395321 |
Synergistic induction of apoptosis by acyclic retinoid and interferon-beta in human hepatocellular carcinoma cells. Obora A,Shiratori Y,Okuno M,Adachi S,Takano Y,Matsushima-Nishiwaki R,Yasuda I,Yamada Y,Akita K,Sano T,Shimada J,Kojima S,Okano Y,Friedman SL,Moriwaki H Hepatology. 2002 Nov;36(5):1115-24 |
| Pubmed id:11983450 |
Acyclic retinoid induces partial differentiation, down-regulates telomerase reverse transcriptase mRNA expression and telomerase activity, and induces apoptosis in human hepatoma-derived cell lines. Yasuda I,Shiratori Y,Adachi S,Obora A,Takemura M,Okuno M,Shidoji Y,Seishima M,Muto Y,Moriwaki H J Hepatol. 2002 May;36(5):660-71 |
| Pubmed id:10766424 |
Involvement of insulin-like growth factor binding protein-3 in the retinoic acid receptor-alpha-mediated inhibition of hepatocellular carcinoma cell proliferation. Murakami K,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I Cancer Lett. 2000 Apr 3;151(1):63-70 |
| Pubmed id:10933057 |
Invasiveness of hepatocellular carcinoma cell lines: contribution of membrane-type 1 matrix metalloproteinase. Murakami K,Sakukawa R,Ikeda T,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I Neoplasia. 1999 Nov;1(5):424-30 |
| Pubmed id:9932610 |
4-[3,5-Bis(trimethylsilyl)benzamido] benzoic acid (TAC-101) inhibits the intrahepatic spread of hepatocellular carcinoma and prolongs the life-span of tumor-bearing animals. Murakami K,Matsuura T,Sano M,Hashimoto A,Yonekura K,Sakukawa R,Yamada Y,Saiki I Clin Exp Metastasis. 1998 Oct;16(7):633-43 |
| Pubmed id:9446792 |
Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1. Tsuji T,Miyazaki M,Fushimi K,Mihara K,Inoue Y,Ohashi R,Ohtsubo M,Hamazaki K,Furusako S,Namba M Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21 |
| Pubmed id:8835345 |
Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines. Tsuboi S,Nagamori S,Miyazaki M,Mihara K,Fukaya K,Teruya K,Kosaka T,Tsuji T,Namba M J Med Virol. 1996 Feb;48(2):133-40 |
| Pubmed id:7524639 |
[A new liver support system composed of functional human cells and a radial-flow bioreactor]. Kawada M,Nagamori S,Aizaki H,Fukaya K,Niiya M,Matsuura T,Sujino H,Hasumura S,Shimizu K,Yoshida H Hum Cell. 1994 Jun;7(2):95-100 |
| Pubmed id:1701409 |
Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines. Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K Hepatogastroenterology. 1990 Oct;37(5):457-60 |
| Pubmed id:2174476 |
[Analysis for the integrated hepatitis B virus genome in cells of established human hepatocellular carcinoma cell line JHH-7]. Fujise K,Nagamori S,Hasumura S,Homma S,Fujita K,Sujino H,Matsuura T,Shimizu K,Niiya M,Ohno T Nihon Shokakibyo Gakkai Zasshi. 1990 Sep;87(9):1851-5 |
| Pubmed id:1707654 |
[Establishment and characterization of a human hepatocellular carcinoma cell line JHH-7 producing alpha -fetoprotein and carcinoembryonic antigen--changes in secretion of AFP and CEA from JHH-7 cells after heat treatment]. Homma S,Nagamori S,Fujise K,Hasumura S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H Hum Cell. 1990 Jun;3(2):152-7 |
| Pubmed id:2856500 |
[Effects of TNF on human hepatocellular carcinoma cell lines and their modification by hyperthermia]. Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Satomi N Hum Cell. 1988 Jun;1(2):238-44 |
| Pubmed id:none |
ヒト肝癌細胞株に対するTumor necrosis factorの影響 蓮村哲 永森静志 藤瀬清隆 本間定 筋野甫 松浦知和 清水恵一郎 新谷稔 里見信子 医学のあゆみ 143(8), 655-656 (1987) |
| Pubmed id:none |
樹立ヒト肝癌株細胞中のB型肝炎ウイルスゲノム 藤瀬 清隆, 藤多 和信, 永森 静志, 蓮村 哲, 本間 定, 筋野 甫, 松浦 知和, 清水 恵一郎, 新谷 稔, 大野 典也, 亀田 治男 肝臓 29(5), 697-698 (1988) |
| Pubmed id:none |
転移の治療実験 村上 孝司 済木育夫 実験医学 16(16), 162-167 (1998) |
| Pubmed id:none |
ヒト肝癌細胞JHH7からの geranylgeranyl pyrophosphate (GGPP) synthase cDNAの単離と発現に関する検討 新規な遺伝子機能アッセイ法の開発 三沢典彦 脂質生化学研究 41, 293-296 (1999) |
| Pubmed id:none |
株化肝細胞癌の遺伝子発現 Gene expression in human hepatocellular carcinoma cell lines 村上 孝司 山田 雄次 済木 育夫 松浦 知和 蓮村 哲 永森 静志 組織培養研究 18(3), 221-228 (1999) |
| Pubmed id:none |
ヒト肝細胞癌由来株JHH-7の分子生物学的特徴とRAR-α特異的結合性を有する化合物TAC-101に対する反応性 大家 真治 村上 孝司 北里 健二 細胞 33(11), 32-35 (2001) |
| Pubmed id:none |
転移の治療実験 村上 孝司 済木育夫 実験医学16(16),162-167 (1988) |
| Pubmed id:none |
ヒト肝細胞癌由来株JHH-7の分子生物学的特徴とRAR-α特異的結合性を有する化合物TAC-101に対する反応性 大家 真治 村上 孝司 北里 健二 細胞 33(11),32-35 (2001) |
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LOT Information
| Cell No. | JCRB1031 | Cell Name | |
|---|---|---|---|
| LOT No. | 10312012 | Lot Specification | distribution |
| Medium | Williams' E medium with 10% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 1.5 - 2.1 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 29 hrs. | Cell Number in Vial (cells/1ml) | 1.9 x 10^6 |
| Viability at cell freezing (%) | 98.4 | Antibiotics Used | free |
| Passage Number | Unknown (10 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Williams' E | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1031 | Cell Name | |
|---|---|---|---|
| LOT No. | 06252008 | Lot Specification | distribution |
| Medium | William's E medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) | Temperature | 37 C |
| Cell Density at Seeding | approx. 2 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. Subculture twice a week. |
| Doubling Time | 30 hrs | Cell Number in Vial (cells/1ml) | 8.8 x 10^6 |
| Viability at cell freezing (%) | 96 | Antibiotics Used | free |
| Passage Number | Unknown (8 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - William's E | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1031 | Cell Name | JHH-7 |
|---|---|---|---|
| LOT No. | 12212002 | Lot Specification | distribution |
| Medium | Williams'E medium with 10% fetal bovine serum (Intergen RB52305). | Temperature | 37 C |
| Cell Density at Seeding | 4.4 x 10^4 cells/sq.cm. | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Medium change once a week. Subculture once a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.1 x 10^6 |
| Viability at cell freezing (%) | 99.0 | Antibiotics Used | free |
| Passage Number | P4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | NT |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1031 | Cell Name | JHH-7 |
|---|---|---|---|
| LOT No. | 01182016 | Lot Specification | distribution |
| Medium | Williams' medium E with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). | Temperature | 37 C |
| Cell Density at Seeding | approx. 2 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 26 hrs. | Cell Number in Vial (cells/1ml) | 4.8 x 10^6 |
| Viability at cell freezing (%) | 95.2 | Antibiotics Used | free |
| Passage Number | Unknown (8 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - Williams' medium E | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 95.2 | Additional information |
| Cell No. | JCRB1031 | Cell Name | JHH-7 |
|---|---|---|---|
| LOT No. | 06252021 | Lot Specification | distribution |
| Medium | William's medium E with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) | Temperature | 37 C |
| Cell Density at Seeding | 0.8 - 2.0 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 22 hrs. | Cell Number in Vial (cells/1ml) | 2.6 x 10^6 |
| Viability at cell freezing (%) | 98 | Antibiotics Used | free |
| Passage Number | Unknown (8 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - William's medium E | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 97 | Additional information |