JCRB0016 P388-D1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0016 Cell Name   P388-D1
Profile   lymphoid neoplasm, derived from P388, (DBA/2 mouse) Other Name   
Animal   mouse Strain   DBA/2
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   neoplasma was induced by MCA Metastasis   
Tissue Metastasized    Genetics   ascitic form of P388
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Used by the NCI cancer chemotherapy screening study.
Classify   tumor Established by   Dawe,C.J. & Potter,M.
Registered by   Sawada,J Regulation for Distribution   
Comment   Release interleukin 1. Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution.
Cell Number on Passage   2.5x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   lymphoid neoplasma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0477
Reference
Pubmed id:3139678Inductive effect of recombinant human interleukin-1 alpha and beta on differentiation of macrophage-like tumor cell line P388D1.
Hanazawa S,Hanaizumi C,Amano S,Hirose K,Ohmori Y,Kumegawa M,Yamaura K,Kitano S
J Cell Physiol. 1988 Sep;136(3):543-6
Pubmed id:3142452Biosynthesis of chondroitin sulfate proteoglycan by P388D1 macrophage-like cell line.
Christner JE
Arteriosclerosis. 1988 Sep-Oct;8(5):535-43
Pubmed id:149171Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line, P388D1. I. Enhancement of LAF production by activated T lymphocytes.
Mizel SB,Oppenheim JJ,Rosenstreich DL
J Immunol. 1978 May;120(5):1497-503
Pubmed id:13671417The amino acid requirements of the Jensen sarcoma in vitro.
McCOY TA,MAXWELL M,KRUSE PF Jr
Cancer Res. 1959 Jul;19(6, Part 1):591-5

Images



LOT Information

Cell No.   JCRB0016 Cell Name   P388-D1
LOT No.   032798 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (ICN Flow 101083). Temperature   37 C
Cell Density at Seeding   3.8x10^4 cells/sq.cm. Methods for Passages   Cells are harvested with 0.25% trypsin and 0.02% EDTA at 37 C for 20 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.9x10^5
Viability at cell freezing (%)   93.9 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Examined.
Chromosome Mode   NT Chromosome Information   Examined.
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0016 Cell Name   P388-D1
LOT No.   020195 Lot Specification   FDSC/distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (BioCell, 4001776) Temperature   37 C
Cell Density at Seeding   3.5x10^4 cells/ml Methods for Passages   Dilution at the density indicated above.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.25x10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,G6PD,MD,MP,PB,AS,LDH
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0016 Cell Name   P388-D1
LOT No.   070292 Lot Specification   distribution
Medium   RPMI1640(lot.051592,062992) + 10% FBS (lot.J.R.S. DO10400, ICN Flow 1010183)+10uM 2-mercaptoethanol Temperature   37 C
Cell Density at Seeding   1-3x10^5 cells/ml Methods for Passages   Dilution is required for subculture. Use 0.25% trypsin with 0.02%EDTA, when adherent cells appeared.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.36x10^5
Viability at cell freezing (%)   89 % Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0016 Cell Name   P388-D1
LOT No.   050791 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum and 2-mercaptoethanol (10uM) Temperature   37 C
Cell Density at Seeding   2.5x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.5x10^5
Viability at cell freezing (%)   76.9 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0016 Cell Name   P388-D1
LOT No.   032390 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum and 10uM 2-mercaptoethanol Temperature   37 C
Cell Density at Seeding   2.5x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0016 Cell Name   P388-D1
LOT No.   040786 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum and 10uM 2-mercaptoethanol Temperature   37 C
Cell Density at Seeding   2.5x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   80% Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0016 Cell Name   P388-D1
LOT No.   11252014 Lot Specification   distribution
Medium   RPMI1640 medium with 10 uM 2-mercaptoethanol and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.1 x 10^5 cells/mL Methods for Passages   Dilution. (Suspension culture.)
Doubling Time   approx. 67 hrs Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   87 Additional information   
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