JCRB0018 J774.1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0018 Cell Name   J774.1
Profile   monocyte-macrophage, IL-1-producing, (BALB/c mouse) Other Name   
Animal   mouse Strain   BALB/c
Genus   Mus Species   musclus
Sex   F Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   reticulum cell sarcoma Metastasis   
Tissue Metastasized    Genetics   Produce large amount of lysozyme and IL-1.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Carry receptors for immunoglobulin and complement. IL-1 productivity is different from the similar cell line, J774A.1 distributed from the ATCC.
Classify   tumor Established by   Ralph,P. & Nakoinz,I.
Registered by   Amano,F. Regulation for Distribution   
Comment    Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4770
Reference
Pubmed id:7543422Improved detection of nitric oxide radical (NO.) production in an activated macrophage culture with a radical scavenger, carboxy PTIO and Griess reagent.
Amano F,Noda T
FEBS Lett. 1995 Jul 24;368(3):425-8
Pubmed id:7729897Intracellular killing of Listeria monocytogenes in the J774.1 macrophage-like cell line and the lipopolysaccharide (LPS)-resistant mutant LPS1916 cell line defective in the generation of reactive oxygen intermediates after LPS treatment.
Inoue S,Itagaki S,Amano F
Infect Immun. 1995 May;63(5):1876-86
Pubmed id:7960121Difference in Legionella pneumophila growth permissiveness between J774.1 murine macrophage-like JA-4 cells and lipopolysaccharide (LPS)-resistant mutant cells, LPS1916, after stimulation with LPS.
Kura F,Suzuki K,Watanabe H,Akamatsu Y,Amano F
Infect Immun. 1994 Dec;62(12):5419-23
Pubmed id:1645329A lipopolysaccharide (LPS)-resistant mutant isolated from a macrophagelike cell line, J774.1, exhibits an altered activated-macrophage phenotype in response to LPS.
Amano F,Akamatsu Y
Infect Immun. 1991 Jun;59(6):2166-74
Pubmed id:1871372[Altered responses of the variants with defective or low epidermal growth factor receptor activity].
Amano F
Tanpakushitsu Kakusan Koso. 1991 May;36(7):978-85
Pubmed id:2110628Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cells.
Bielecki J,Youngman P,Connelly P,Portnoy DA
Nature. 1990 May 10;345(6271):175-6
Pubmed id:3417786A prelysosomal compartment sequesters membrane-impermeant fluorescent dyes from the cytoplasmic matrix of J774 macrophages.
Steinberg TH,Swanson JA,Silverstein SC
J Cell Biol. 1988 Sep;107(3):887-96
Pubmed id:318922Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture.
Ralph P,Nakoinz I
Cancer Res. 1977 Feb;37(2):546-50
Pubmed id:1101071Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line.
Ralph P,Nakoinz I
Nature. 1975 Oct 2;257(5525):393-4


Research results by users. Click！
Pubmed id:29894908 Differences in interleukin-1β release-inducing activity of Candida albicans toward dendritic cells and macrophages.
Hasebe A,Saeki A,Yoshida Y,Shibata KI
Arch Oral Biol. 2018 Sep;93():115-125

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LOT Information

Cell No.   JCRB0018 Cell Name   
LOT No.   09022008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. No. 10099. Lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0018 Cell Name   J774.1
LOT No.   112592 Lot Specification   distribution
Medium   RPMI1640 medium (090892) with 10% fetal calf serum(Hyclone 1115972). Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   Cells are washed once with PBS and harvested aftertreatment with 0.02%EDTA & 0.25%trypsin(37C,15min)
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.2x10^6
Viability at cell freezing (%)   93.1 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=66 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0018 Cell Name   J774.1
LOT No.   050291 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding   1x10^6 /dish Methods for Passages   0.02 % EDTA + 0.25 % trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0018 Cell Name   J774.1
LOT No.   100689 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.9x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0018 Cell Name   J774.1
LOT No.   012386 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0018 Cell Name   
LOT No.   031797 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1.0-2.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   81.0 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0018 Cell Name   J774.1
LOT No.   01162014 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.1 - 4.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   95.4 Additional information   
Cell No.   JCRB0018 Cell Name   J774.1
LOT No.   10262016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.5 - 5.4 x 10^5 cells/mL Methods for Passages   Cells were harvested by pipetting, or by treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   4.3 x 10^6
Viability at cell freezing (%)   81.9 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   81.9 Additional information   
Cell No.   JCRB0018 Cell Name   J774.1
LOT No.   10242017 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.1 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   5.2 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
Cell No.   JCRB0018 Cell Name   J774.1
LOT No.   09162020 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.2 - 2.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 23 hrs. Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI-1640 medium CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
Cell No.   JCRB0018 Cell Name   J774.1
LOT No.   04272023 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   1 -. 5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Sometimes, the cells may strongly attach to substratum. In such case, please use scraping.
Doubling Time   37 C Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
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