JCRB0033 CCRF-CEM

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0033 Cell Name   CCRF-CEM
Profile   leukemia, acute T lymphoblastic Other Name   CEM
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   4
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute lymphoblastoid leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Near diploid, no Ig-secreting, free of virus particle.
Classify   tumor Established by   Foley,G.E.
Registered by   Sofuni,T. Regulation for Distribution   free
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution.
Cell Number on Passage   3-5 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood (buffy coat)
Tissue Type   T lymphoblastoid leukemia

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0207
Reference
Pubmed id:3493047T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.
Drexler HG,Gaedicke G,Minowada J
Blut. 1987 Feb;54(2):79-87
Pubmed id:6312323Molecular characterization of genome of a novel human T-cell leukaemia virus.
Chen IS,McLaughlin J,Gasson JC,Clark SC,Golde DW
Nature. 1983 Oct 6-12;305(5934):502-5
Pubmed id:841332Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8
Pubmed id:4170381Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma.
Adams RA
Cancer Res. 1967 Dec;27(12):2479-82
Pubmed id:4295047Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster.
Adams RA,Foley GE,Uzman BG,Farber S,Lazarus S,Kleinman L
Cancer Res. 1967 Apr;27(4):772-83
Pubmed id:5224274Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope.
Uzman BG,Foley GE,Farber S,Lazarus H
Cancer. 1966 Nov;19(11):1725-42
Pubmed id:5320250Cytogenetic analysis of human lymphoblasts in continuous culture.
McCarthy RE,Junius V,Farber S,Lazarus H,Foley GE
Exp Cell Res. 1965 Oct;40(1):197-200

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LOT Information

Cell No.   JCRB0033 Cell Name   CCRF-CEM
LOT No.   10242012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3 - 5 x 10^5 cells/mL Methods for Passages   Dilution (suspension culture)
Doubling Time   approx. 27 hrs Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   93.6 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   Yes
Viability immediately after thawing (%)    Additional information   Mislabeled as CCRF-CKM on ample.
Cell No.   JCRB0033 Cell Name   CCRF-CEM
LOT No.   011994 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat inactivated fetal bovine serum. Temperature   37 C
Cell Density at Seeding   1-5x10^5 cells/ml Methods for Passages   Dilution into a fresh medium.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.9x10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,PepB
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0033 Cell Name   CCRF-CEM
LOT No.   091790 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   3-5 x 10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   36 hr Cell Number in Vial (cells/1ml)   3.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:11,12
D7S820:9,12
D16S539:10,13
VWA:17,19
TH01:6,7
AM:X
TPOX:8
CSF1PO:11
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0033 Cell Name   CCRF-CEM
LOT No.   111285 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   3-5 x 10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0033 Cell Name   CCRF-CEM
LOT No.   08052021 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   2.0-8.0x10^4 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.88x10^6
Viability at cell freezing (%)   98.74 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11,12
D7S820:9,12
D16S539:10,13
VWA:17,19
TH01:6,7
AM:X
TPOX:8
CSF1PO:11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   80 Additional information   
Images
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