JCRB1032 OZ

Cell information

Important Notice(s)

On the agreement for distribution of JHH series cell lines, NOZ and OZ

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1032 Cell Name   OZ
Profile   Human bile duct cell line established from ascites of the tumor patient who had differentiated adenocarcinoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   71
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   differentiated adenocarcinoma papilla and poorly differentiated adenocarcinoma. CEA 8.0 ng/ml. Metastasis   Yes
Tissue Metastasized   ascites Genetics   No. of chromosome, 51. Td=48 hrs.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells were established from ascites of the patient whose bile duct was emphraxed by mucinous materials produced by adenocarcinoma cells. Cells produce CEA, BMG. High levels of P1-P, r-GTP, LDH were observed in cells. PAS (+). Transplantalbe to nude mice.
Classify   tumor Established by   Honma,S. & Nagamori,S.
Registered by   Nagamori,S. Regulation for Distribution   Require negotiation and agreement from Dr.Nagamori before shipping.
Comment   Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. (r-GPT = gamma GPT) Year   2001
Medium   Williams'E medium with 10% fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3118
Reference
Pubmed id:2889643 Human bile duct carcinoma cell line producing abundant mucin in vitro.
Homma S,Nagamori S,Fujise K,Yamazaki K,Hasumura S,Sujino H,Matsuura T,Shimizu K,Kameda H,Takaki K
Gastroenterol Jpn. 1987 Aug;22(4):474-9


Research results by users. Click!
Pubmed id:22639408 Twisted gastrulation expression in cholangiocellular and hepatocellular carcinoma.
Johnston J,Al-Bahrani R,Abuetabh Y,Chiu B,Forsman CL,Nagamori S,Leng R,Petryk A,Sergi C
J Clin Pathol. 2012 Oct;65(10):945-8
Pubmed id:23084587 Overexpression of epithelial-mesenchymal transition-related markers according to cell dedifferentiation: clinical implications as an independent predictor of poor prognosis in cholangiocarcinoma.
Ryu HS,Chung JH,Lee K,Shin E,Jing J,Choe G,Kim H,Xu X,Lee HE,Kim DG,Lee H,Jang JJ
Hum Pathol. 2012 Dec;43(12):2360-70
Pubmed id:22457476 Therapeutic potential of amanitin-conjugated anti-epithelial cell adhesion molecule monoclonal antibody against pancreatic carcinoma.
Moldenhauer G,Salnikov AV,Lüttgau S,Herr I,Anderl J,Faulstich H
J Natl Cancer Inst. 2012 Apr 18;104(8):622-34
Pubmed id:22309595 Fibroblast growth factor 19 expression correlates with tumor progression and poorer prognosis of hepatocellular carcinoma.
Miura S,Mitsuhashi N,Shimizu H,Kimura F,Yoshidome H,Otsuka M,Kato A,Shida T,Okamura D,Miyazaki M
BMC Cancer. 2012 Feb 6;12():56
Pubmed id:22075503 Expression of E-cadherin and β-catenin in two cholangiocarcinoma cell lines (OZ and HuCCT1) with different degree of invasiveness of the primary tumor.
Abuetabh Y,Persad S,Nagamori S,Huggins J,Al-Bahrani R,Sergi C
Ann Clin Lab Sci. 2011 Summer;41(3):217-23
Pubmed id:20922056 Mesothelin as a potential therapeutic target in human cholangiocarcinoma.
Yu L,Feng M,Kim H,Phung Y,Kleiner DE,Gores GJ,Qian M,Wang XW,Ho M
J Cancer. 2010 Oct 1;1():141-9
Pubmed id:20044606 Infrequent amplification of JUN in hepatocellular carcinoma.
Endo M,Yasui K,Nakajima T,Gen Y,Tsuji K,Dohi O,Zen K,Mitsuyoshi H,Minami M,Itoh Y,Taniwaki M,Tanaka S,Arii S,Okanoue T,Yoshikawa T
Anticancer Res. 2009 Dec;29(12):4989-94
Pubmed id:19319137 Vandetanib (ZD6474), an inhibitor of VEGFR and EGFR signalling, as a novel molecular-targeted therapy against cholangiocarcinoma.
Yoshikawa D,Ojima H,Kokubu A,Ochiya T,Kasai S,Hirohashi S,Shibata T
Br J Cancer. 2009 Apr 21;100(8):1257-66
Pubmed id:19453019 Rapamycin inhibits growth of cholangiocarcinoma cells.
Okada T,Sawada T,Kubota K
Hepatogastroenterology. 2009 Jan-Feb;56(89):6-10
Pubmed id:18442209 Effect of histone deacetylase inhibitor on proliferation of biliary tract cancer cell lines.
Xu LN,Wang X,Zou SQ
World J Gastroenterol. 2008 Apr 28;14(16):2578-81
Pubmed id:19383345 Exon 2 deletion splice variant of gamma-glutamyl carboxylase causes des-gamma-carboxy prothrombin production in hepatocellular carcinoma cell lines.
Ueda N,Shiraha H,Fujikawa T,Takaoka N,Nakanishi Y,Suzuki M,Matsuo N,Tanaka S,Nishina S,Uemura M,Takaki A,Shiratori Y,Yamamoto K
Mol Oncol. 2008 Oct;2(3):241-9
Pubmed id:15492814 Anti-endostatin monoclonal antibody enhances growth of human hepatocellular carcinoma cells by inhibiting activity of endostatin secreted by the transplanted cells in nude mice.
Tsuboi S,Nouso K,Tomono Y,Nagamori S,Naito I,Watanabe T,Manabe K,Ohmori H,Nakatsukasa H,Miyazaki M,Higashi T,Shiratori Y,Namba M,Ninomiya Y
Int J Oncol. 2004 Nov;25(5):1267-71
Pubmed id:12654561 Development of a therapeutic adenoviral vector for cholangiocarcinoma combining tumor-restricted gene expression and infectivity enhancement.
Nagi P,Vickers SM,Davydova J,Adachi Y,Takayama K,Barker S,Krasnykh V,Curiel DT,Yamamoto M
J Gastrointest Surg. 2003 Mar-Apr;7(3):364-71
Pubmed id:9446792 Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1.
Tsuji T,Miyazaki M,Fushimi K,Mihara K,Inoue Y,Ohashi R,Ohtsubo M,Hamazaki K,Furusako S,Namba M
Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21
Pubmed id:9135018 Tamoxifen-mediated growth inhibition of human cholangiocarcinoma.
Sampson LK,Vickers SM,Ying W,Phillips JO
Cancer Res. 1997 May 1;57(9):1743-9
Pubmed id:8835345 Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
Tsuboi S,Nagamori S,Miyazaki M,Mihara K,Fukaya K,Teruya K,Kosaka T,Tsuji T,Namba M
J Med Virol. 1996 Feb;48(2):133-40
Pubmed id:1701409 Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:2889643 Human bile duct carcinoma cell line producing abundant mucin in vitro.
Homma S,Nagamori S,Fujise K,Yamazaki K,Hasumura S,Sujino H,Matsuura T,Shimizu K,Kameda H,Takaki K
Gastroenterol Jpn. 1987 Aug;22(4):474-9
Pubmed id:3005723 [Effects of hyperthermia on the proliferation and viability of cultured human hepatobiliary carcinoma cells].
Homma S,Nagamori S,Fujise K,Sujino H,Matsuura T,Simizu K,Kameda H
Nihon Shokakibyo Gakkai Zasshi. 1985 Sep;82(9):2043-8
Pubmed id:20565817

. 0;():
Pubmed id:none 高分化型ヒト不死化肝細胞株(OUMS‐29)と肝癌細胞株(JHH‐1)でのエンドスタチン分泌の相違
友野靖子 安藤香織 内藤一郎 佐渡義一 坪井壮 永森静志 宮崎正博 沖垣達 二宮善文
重井医学年報 24, 7-14 (2003)
Pubmed id:none 樹立ヒト肝癌株細胞中のB型肝炎ウイルスゲノム
藤瀬 清隆, 藤多 和信, 永森 静志, 蓮村 哲, 本間 定, 筋野 甫, 松浦 知和, 清水 恵一郎, 新谷 稔, 大野 典也, 亀田 治男
肝臓 29(5), 697-698 (1988)
Pubmed id:none ヒト肝胆道系癌培養細胞の生存と増殖に及ぼす温熱の効果
本間 定, 永森 静志, 藤瀬 清隆, 筋野 甫, 松浦 知和, 清水 恵一郎, 亀田 治男
肝臓 24(5), 569 (1983)

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LOT Information

Cell No.   JCRB1032 Cell Name   
LOT No.   05222012 Lot Specification   distribution
Medium   Williams' E medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   3 - 4 days Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Williams' E CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1032 Cell Name   
LOT No.   03192007 Lot Specification   distribution
Medium   William's E medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA, passage once a week. Cells attached hard to dishes and trypsinization at 37 C for 20-30 minutes required.
Doubling Time   80 hrs Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   98.3 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP and G6PD, LD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - William's E medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1032 Cell Name   OZ
LOT No.   02212003 Lot Specification   distribution
Medium   Williams'E medium with 10% fetal calf serum(Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.2 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.2% trypsin. Medium change twice a week and subculture every 10 days..
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(not shown), NP, LDH examined. Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   NT
Additional information   
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