JCRB1039 SKN-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1039 Cell Name   SKN-3
Profile   Human oral squamous carcinoma established from xenotransplantation in mice. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   oral
Case history   oral squamous carcinoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells were estabished through xenotransplanted tumor in nude mice
Classify   tumor Established by   Yanagihara,K.
Registered by   Yanagihara,K. Regulation for Distribution   
Comment    Year   2003
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   1.7 x 10^6 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   oral cavity
Tissue Type   squamous carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3168
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1299059
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LOT Information

Cell No.   JCRB1039 Cell Name   
LOT No.   09012008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   4 - 7 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 40 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1039 Cell Name   SKN-3
LOT No.   10112019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   4.1 - 7.5 x 10^4 cells.mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
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