JCRB1040 FF101

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1040 Cell Name   FF101
Profile   Rat hepatoma cell line with producing unidentified growth factor maintained in serum-free RPMI1640 medium for 444 passages. Other Name   
Animal   rat Strain   donryu
Genus   Rattus Species   norvegicus
Sex    Age   adult
Identity    Tissue for Primary Cancer   liver
Case history   hepatoma was induced by feeding 0.06% 3'-methyl-4-dimethylaminoazobenzene for five months. Metastasis   
Tissue Metastasized    Genetics   Cells have characteristics of hepatoma and cholangioma.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   The cells are expected to produce FF-GF (unidentified growth factor, 70KDa). Sup. of the cell culture have growth activating activity(AH66, K562, Y3, YAC-1,BALB/c,3T3,NS-1). Cells have been cultured with serum-free medium from the primary cuture.
Classify   tumor Established by   Matsumoto,H.et.al.
Registered by   Matsumoto,H Regulation for Distribution   not defined
Comment   Cells have been maintained by continuous cell culture without freezing at all. Year   2002
Medium   Serum-free, protein-free RPMI1640 with increased concentration of glutamine (double). Methods for Passages   Adhesion to dishes a weak. Pipetting with 2-3 times is enough to harvest cells. Subculture once every 7-10 days.
Cell Number on Passage   not defined. Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8562
Pubmed id:1281996Purification and characterization of a novel growth factor (FF-GF) synthesized by a rat hepatoma cell line, FF101.
Matsuda H,Matsumoto M,Kitahara H,Haraguchi S,Kanai K
Biochem Biophys Res Commun. 1992 Dec 15;189(2):654-61
Pubmed id:2702652Partial purification of a growth factor synthesized by a rat hepatoma cell line established in serum-free medium.
Matsuda H,Matsumoto M,Haraguchi S,Kanai K
Cancer Res. 1989 Apr 15;49(8):2118-22


LOT Information

Cell No.   JCRB1040 Cell Name   FF101
LOT No.   10282003 Lot Specification   distribution
Medium   Serum-free, protein-free RPMI1640 with increased concentration of glutamine (double). Temperature   37C
Cell Density at Seeding   3.4 x 10^4 cells/sq.cm Methods for Passages   Pipetting with 2-3 times is enough to harvest cells.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   86.2 Antibiotics Used   Free
Passage Number   P455 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium + FM-1 (1 to 1 mix) CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information