JCRB1006.1 OUMS-36T-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1006.1 Cell Name   OUMS-36T-1
Profile   hTRT gene transfected normal human embryo fibroblast cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   embryo
Case history   normal transfected with hTRT gene Metastasis   
Tissue Metastasized    Genetics   10^5 cells/well were transfected with pMXpuro-hTRT (50ng/ml) 3 times with the electroporation.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   normal human embryo cells transfected by hTRT gene (pMXpuro).
Classify   transformed Established by   Kouchi,Y. & Namba,M.
Registered by   Namba,M. Regulation for Distribution   
Comment    Year   2001
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   0.1% trypsin and 0.02% EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3092
Reference
Pubmed id:noneNormal huma fibroblast cell lines tranfected with the hTRT gene -OUMS36T cell line series-
Kouchi,H. and Namba,M.
Tiss. Cult. Res. Commun., 19: 203-204, 2000.

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LOT Information

Cell No.   JCRB1006.1 Cell Name   
LOT No.   04182012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   6 - 10 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 25 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   P49 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1006.1 Cell Name   OUMS-36T-1
LOT No.   02222003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Mitsubishi PFCS, PVF01). Temperature   37 C
Cell Density at Seeding   7.7 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.1% trypsin and 0.02% EDTA at room temperature for 5 min. Medium change once a week. Subculture once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   P46 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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