JCRB1006.3 OUMS-36T-3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1006.3 | Cell Name | OUMS-36T-3 |
|---|---|---|---|
| Profile | hTRT gene transfected normal human embryo fibroblast cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | embryo |
| Case history | normal transfected with hTRT gene | Metastasis | |
| Tissue Metastasized | Genetics | 10^6 cells/well were transfected with pMXpuro-hTRT (100ng/ml) twice with electroporation. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | normal human embryo cells transfected by hTRT gene (pMXpuro). |
| Classify | transformed | Established by | Kouchi,Y. & Namba,M. |
| Registered by | Namba,M. | Regulation for Distribution | |
| Comment | Year | 2001 | |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum. | Methods for Passages | 0.1% trypsin and 0.02% EDTA. |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5 % | Tissue Sampling | embryo |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:none | Normal huma fibroblast cell lines tranfected with the hTRT gene -OUMS36T cell line series- Kouchi,H. and Namba,M. Tiss. Cult. Res. Commun., 19: 203-204, 2000. |
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LOT Information
| Cell No. | JCRB1006.3 | Cell Name | OUMS-36T-3 |
|---|---|---|---|
| LOT No. | 06272003 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bvine serum (Mitsubishi, PVF01). | Temperature | 37 C |
| Cell Density at Seeding | 9.1 x 10^3 cells/sq.cm. | Methods for Passages | Cells are harvested after treatment with 0.1 % trypsin and 0.02% EDTA at room temperature for 3 minutes. Subculture once a week and medium change once a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.5 x 10^6 |
| Viability at cell freezing (%) | 90.4 | Antibiotics Used | free |
| Passage Number | P48 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Under examination. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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