JCRB1041 SEKI

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1041 Cell Name   SEKI
Profile   Human malignant melanoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   28
Identity   available Tissue for Primary Cancer   melanoma
Case history   malignant melanoma Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Cells are consisted from both melanin containig and melanin non-containing cells. Cells can be cloned by soft agar method. Melanin granules are observed in cells.
Classify   tumor Established by   Shimoyama,M.
Registered by   Nose,K. Regulation for Distribution   
Comment    Year   2002
Medium   RPMI1640 medium with 20% fetal bovine serum. Methods for Passages   Dilution. Td = 4-5 days.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin of lower leg
Tissue Type   melanoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3162
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LOT Information

Cell No.   JCRB1041 Cell Name   SEKI
LOT No.   05022003 Lot Specification   distribution
Medium   RPMI 1640 medium with 20% fetal bovine serum Temperature   37 C
Cell Density at Seeding    Methods for Passages   Vigorous pipetting and dilution (most of cells are suspension and some cells adhere to substratum).
Doubling Time    Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   80.5 Antibiotics Used   free
Passage Number   P18* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LDH.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   80.5 Additional information   
Cell No.   JCRB1041 Cell Name   SEKI
LOT No.   10292020 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO:catalog No.11875) with 20% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   1.17-1.22x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.19x10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   p21* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,11
D13S317:8,9
D7S820:9,12
D16S539:9,10.1,11
VWA:16,18
TH01:9
AM:X
TPOX:11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   79.1 Additional information   
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