JCRB0071 BALL-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0071 | Cell Name | BALL-1 |
|---|---|---|---|
| Profile | Acute lymphoblastoid leukemia (ALL) cell line from human. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | acute lymphoblastoid leukemia (ALL) | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | |
| Classify | tumor | Established by | Hiraki,S. |
| Registered by | Akiyama,M. | Regulation for Distribution | free |
| Comment | Year | 1985 | |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | Dilution, twice a week. |
| Cell Number on Passage | split ratio=1/3 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type | ALL |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2698048 | Expression of cytokine genes in hematological malignancies. Okamura S,Hayashi S,Kawasaki C,Kondo S,Asano Y,Omori F,Otsuka T,Shibuya T,Harada M,Niho Y Nihon Ketsueki Gakkai Zasshi. 1989 Dec;52(8):1423-32 |
| Pubmed id:197412 | Human B cell, T cell and null cell leukaemic cell lines derived from acute lymphoblastic leukaemias. Miyoshi I,Hiraki S,Tsubota T,Kubonishi I,Matsuda Y,Nakayama T,Kishimoto H,Kimura I,Masuji H Nature. 1977 Jun 30;267(5614):843-4 |
| Images |
|---|
  |
LOT Information
| Cell No. | JCRB0071 | Cell Name | |
|---|---|---|---|
| LOT No. | 09212012 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 3.4 x 10^5 cells/mL | Methods for Passages | Simple dilution. (suspension culture) |
| Doubling Time | approx. 28 hrs | Cell Number in Vial (cells/1ml) | 1.1 x 10^6 |
| Viability at cell freezing (%) | 82.9 | Antibiotics Used | free |
| Passage Number | Unknown (10 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0071 | Cell Name | BALL-1 |
|---|---|---|---|
| LOT No. | 053193 | Lot Specification | distribution |
| Medium | RPMI-1640 with 10% heat inactivated fetal bovine serum (Hyclone 115975) | Temperature | 37 C |
| Cell Density at Seeding | 3.5x10^5 cells/ml | Methods for Passages | Harvest cells by centrifuge and wash cells. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.75x10^4 |
| Viability at cell freezing (%) | 73.9 | Antibiotics Used | free |
| Passage Number | P7 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0071 | Cell Name | BALL-1 |
|---|---|---|---|
| LOT No. | 042190 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | dilution 1/3 | Methods for Passages | Dilution, twice a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.2x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P5* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0071 | Cell Name | BALL-1 |
|---|---|---|---|
| LOT No. | 100187 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | dilution 1/3 | Methods for Passages | Dilution, twice a week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 10^6 |
| Viability at cell freezing (%) | 92% | Antibiotics Used | free |
| Passage Number | P4* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD,LD,NP |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |