JCRB0075 SF-TY

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0075 Cell Name   SF-TY
Profile   normal fibroblast Other Name   Takahashi
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   skin
Case history   normal Metastasis   
Tissue Metastasized    Genetics   normal
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   normal skin fibroblast
Classify   normal Established by   Akiyama,M.
Registered by   Akiyama,M. Regulation for Distribution   free
Comment   HLA:A2,A24,B5,Bw22 Year   1985
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/3 Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3163
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LOT Information

Cell No.   JCRB0075 Cell Name   
LOT No.   07162010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 0.1 mM non-essential amino acids and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.8 - 3.8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 42 hrs Cell Number in Vial (cells/1ml)   7.3 x 10^5
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0075 Cell Name   
LOT No.   07092007 Lot Specification   distribution
Medium   Eagle's minimam essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   2-5 x10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   7.6 x 10^5
Viability at cell freezing (%)   85.3 Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   083093 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% heat inactivated FBS(ICN) Temperature   37C
Cell Density at Seeding   5-10x10^5 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.3x10^5
Viability at cell freezing (%)   94.9 Antibiotics Used   free
Passage Number   P11 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   031692 Lot Specification   distribution
Medium   MEM with NEAA + FCS 10% Temperature   37 C
Cell Density at Seeding   dilution 6.2x10^5 Methods for Passages   0.02 % EDTA + 0.25 % trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   1x10^6
Viability at cell freezing (%)   85.2 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   091190 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.9x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P8 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   082389 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   062487 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   04042014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.5 - 4.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   6.2 x 10^5
Viability at cell freezing (%)   82.0 Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:8,12
D7S820:11
D16S539:9,11
VWA:16,17
TH01:8,9
AM:X,Y
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   82.0 Additional information   
Cell No.   JCRB0075 Cell Name   
LOT No.   102198 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essent- ial amino acids and 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   4-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 40 hrs Cell Number in Vial (cells/1ml)   8.7 x 10^5
Viability at cell freezing (%)   85.0 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   10192015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.6 - 3.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   34 - 54 hrs Cell Number in Vial (cells/1ml)   8.1 x 10^5
Viability at cell freezing (%)   87.1 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   87.1 Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   03272017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.9 - 3.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 52 hrs. Cell Number in Vial (cells/1ml)   7.1 x 10^5 cells/mL
Viability at cell freezing (%)   87.7 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   87.7 Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   08082018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non essential amino acids (NEAA) and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.9 - 3.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   5.0 x 10^5
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.3 Additional information   
Cell No.   JCRB0075 Cell Name   SF-TY
LOT No.   12202021 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Biowest Cat. # S1800-500) Temperature   37 C
Cell Density at Seeding   2.3 - 2.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   7.3 x 10^5
Viability at cell freezing (%)   80.6 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   81.3 Additional information   Some commercially available EMEM with NEAA does not contain L-glutamine (one of essential component of EMEM). In such case, please add L-glutamine to 2 mM.
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