JCRB0077 PC-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0077 Cell Name   PC-3
Profile   adenocarcinoma, intermediately differentiated Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   48
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung adeno carcinoma Metastasis   
Tissue Metastasized    Genetics   intermediately differentiated
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   notice:different cell line from PC-3 ATCC CRL1435
Classify   tumor Established by   K.Tsuji et.al.
Registered by   Akiyama,M. Regulation for Distribution   
Comment   Mycoplasma contamination was detected when submitted at NIHS and eliminated at 1986. Year   1985
Medium   Eagle's minimal essential medium with 10% heat inactivated fetal bovine serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/3 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_S982
Reference
Pubmed id:4771542[Therapeutic difficulties in supraventricular tachycardia without any evident changes in the heart].
Janukowicz C
Pol Tyg Lek. 1973 Nov 19;28(47):1861-2

Images



LOT Information

Cell No.   JCRB0077 Cell Name   PC-3
LOT No.   09302011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat-inactivated fetal bovine serum (FBS, GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   approx. 6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage approx. once a week.
Doubling Time   approx. 45 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   94.8 Antibiotics Used   free
Passage Number   16* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0077 Cell Name   
LOT No.   01072008 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat- inactivated FBS (lot; ) Temperature   37 C
Cell Density at Seeding   2.0 x 10^5cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   approx. 90hr Cell Number in Vial (cells/1ml)   5.6x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0077 Cell Name   PC-3
LOT No.   110794 Lot Specification   distribution
Medium   Eagle's minimam essential medium with 10% heat inactivated fetal bovine serum (Hyclone lot.11151031) Temperature   37 C
Cell Density at Seeding   2.5-3x10^6 cells/sq.cm. Methods for Passages   Wash with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5-15min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.35x10^6
Viability at cell freezing (%)   96.7 Antibiotics Used   free
Passage Number   P18* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0077 Cell Name   PC-3
LOT No.   100594 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat inactivated fetal bovine serum (J.R.Sci.DO10400) Temperature   37 C
Cell Density at Seeding   2x10^6 cells/10cm dish Methods for Passages   Cells are harvested by treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.24x10^6
Viability at cell freezing (%)   97.9 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   + Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0077 Cell Name   PC-3
LOT No.   081594 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10%heat-inactivated fetal bovine serum/Flow0070893,JRS.CO19407 Temperature   37 C
Cell Density at Seeding   1x10^6 cells/10cm dish Methods for Passages   Cells were treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.3x10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   + Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0077 Cell Name   PC-3
LOT No.   021288 Lot Specification   distribution
Medium   MEM + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P15* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0077 Cell Name   
LOT No.   060399 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat- inactivated FBS (lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   P17* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0077 Cell Name   PC-3
LOT No.   07162019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.1 - 2.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   2 - 4 days Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   Unknown (17 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93.2 Additional information   
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