JCRB0094 P30/OHK

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0094 Cell Name   P30/OHK
Profile   leukemia, acute non T non B lymphoblastic Other Name   OHK
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   11
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   non T non B acute lymphoblastic leukemia (ALL) Metastasis   
Tissue Metastasized    Genetics   CD5-,CD2-,Ia+,CD20-(revised to be negative at 2009/1/22),CD19+,J5+,TdT+,EBNA-,CD8+/-
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   PAS+,AcPase+,having pre B characteristic
Classify   tumor Established by   Hirose,M.
Registered by   Minato,K. Regulation for Distribution   Permission required. No commercial purpose.
Comment   CD8 antigen is induced by addition of TPA. Pre-preB ALL strain. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution every 4 days.
Cell Number on Passage   10^5 cells/60mm dish Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1631
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909252
Reference
Pubmed id:6983990A novel pre-T cell line derived from acute lymphoblastic leukemia.
Hirose M,Minato K,Tobinai K,Ohira M,Ise T,Watanabe S,Shimoyama M,Taniwaki M,Abe T
Gan. 1982 Aug;73(4):600-5

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LOT Information

Cell No.   JCRB0094 Cell Name   P30/OHK
LOT No.   111092 Lot Specification   distribution
Medium   RPMI1640 medium (090892) with 10% fetal bovine serum (JRHB 1B1062) Temperature   37 C
Cell Density at Seeding   1.7x10^5 cells/ml Methods for Passages   suspension culture, dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.2x10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   P14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=44 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0094 Cell Name   P30/OHK
LOT No.   011789 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   10^5 cells/60mm dish Methods for Passages   Dilution every 4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P14* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:10
D7S820:10,11
D16S539:9
VWA:14,21
TH01:9
AM:X
TPOX:10,11
CSF1PO:9,11
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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