JCRB1046 OVSAHO Cell information
Cell type:general cells
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JCRB No.
JCRB1046
Cell Name
OVSAHO
Profile
Human cell line derived from ovarian tumour.
Other Name
Animal
human
Strain
Genus
Homo
Species
sapiens
Sex
F
Age
56
Identity
available
Tissue for Primary Cancer
ovary
Case history
ovarian carcinoma, stage IIIc, FAMT(15) CFF(6) treatment done previously.
Metastasis
Yes
Tissue Metastasized
abdominal metastasis
Genetics
PR(-) / tumour markers, SLX, TPA(x).
Life Span
infinite
Crisis PDL
Morphology
epithelial-like
Character
Cells are transplantable to both mouse hypodermis and abdominal.
Classify
tumour
Established by
Gorai,I. et.al.
Registered by
Gorai,I.
Regulation for Distribution
Comment
Td=18.3 hours
Year
2002
Medium
RPMI1640 medium with 10% fetal bovine serum.
Methods for Passages
Cells are harvested after treatment with 0.05% trypsin. Change medium every 3-4 days.
Cell Number on Passage
5 x 10^4 cells/ml
Race
Japanese
CO2 Conc.
5 %
Tissue Sampling
abdominal metastatic focus
Tissue Type
adenocarcinoma
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
tested
Detected RNA Virus
tested
CMV
-
parvoB19
-
HCV
-
HTLV-1
-
EBV
-
HBV
-
HIV-1
-
HTLV-2
-
HHV6
-
HTLV-1
-
HIV-2
-
HAV
-
HHV7
-
HTLV-2
-
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV
-
HIV-1
-
JCV
-
HIV-2
-
ADV
-
HPV18
-
Notes
Reference
Pubmed id:11121861 Cyclophosphamide and 5-fluorouracil act synergistically in ovarian clear cell adenocarcinoma cells. Ohta I,Gorai I,Miyamoto Y,Yang J,Zheng JH,Kawata N,Hirahara F,Shirotake S Cancer Lett. 2001 Jan 10;162(1):39-48
Pubmed id:9328139 Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20. Yanagibashi T,Gorai I,Nakazawa T,Miyagi E,Hirahara F,Kitamura H,Minaguchi H Br J Cancer. 1997;76(7):829-35
Pubmed id:7535723 Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties. Gorai I,Nakazawa T,Miyagi E,Hirahara F,Nagashima Y,Minaguchi H Gynecol Oncol. 1995 Apr;57(1):33-46
Pubmed id:7882618 Marked induction of gelatinases, especially type B, in host fibroblasts by human ovarian cancer cells in athymic mice. Miyagi E,Yasumitsu H,Hirahara F,Nagashima Y,Minaguchi H,Miyazaki K,Umeda M Clin Exp Metastasis. 1995 Mar;13(2):89-96
LOT Information
Cell No.
JCRB1046
Cell Name
OVSAHO
LOT No.
09282010
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099)
Temperature
37 C
Cell Density at Seeding
1 - 3 x 10^5 cells/ml
Methods for Passages
Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.
Doubling Time
approx. 3 days
Cell Number in Vial (cells/1ml)
2.9 x 10^6
Viability at cell freezing (%)
92.0
Antibiotics Used
free
Passage Number
P43
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as human by NP, G6PD (type N), MD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
D5S818:12,13 D13S317:8 D7S820:8,10 D16S539:9 VWA:14 TH01:6 AM:X TPOX:8,11 CSF1PO:10,12
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI 1640
CO2 Conc.
5%
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB1046
Cell Name
OVSAHO
LOT No.
03252003
Lot Specification
distribution
Medium
RPMI1640 medium with 10% fetal calf serum (Intergen RB52305).
Temperature
37 C
Cell Density at Seeding
4.5 x 10^4 cells/sq.cm.
Methods for Passages
Cells harvested after treatment with 0.05% trypsin. Medium change twice a week and subculture once a week.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
4.5 x 10^6
Viability at cell freezing (%)
94.0
Antibiotics Used
free
Passage Number
P41
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
G6PD(type B), NP, LDH examined. Human not HeLa.
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
Culture medium containing 5% DMSO.
CO2 Conc.
5 %
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB1046
Cell Name
OVSAHO
LOT No.
04062015
Lot Specification
distribution
Medium
RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091).
Temperature
37 C
Cell Density at Seeding
2.7 - 4.2 x 10^5 cells/ml
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 43 hrs
Cell Number in Vial (cells/1ml)
4.1 x 10^6
Viability at cell freezing (%)
86.0
Antibiotics Used
free
Passage Number
P44
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI1640
CO2 Conc.
5%
Viability immediately after thawing (%)
86.0
Additional information
Cell No.
JCRB1046
Cell Name
OVSAHO
LOT No.
12012017
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012).
Temperature
37 C
Cell Density at Seeding
1.0 - 1.7 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
approx. 43 hrs.
Cell Number in Vial (cells/1ml)
2.4 x 10^6
Viability at cell freezing (%)
98.0
Antibiotics Used
free
Passage Number
P43
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DSMO, 20% FBS - RPMI1640
CO2 Conc.
5%
Viability immediately after thawing (%)
98.0
Additional information
Cell No.
JCRB1046
Cell Name
OVSAHO
LOT No.
09012023
Lot Specification
distribution
Medium
RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322).
Temperature
37 C
Cell Density at Seeding
1.8 - 2.0 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.05% trypsin.
Doubling Time
44 - 62 hrs.
Cell Number in Vial (cells/1ml)
4.0 x 10^6
Viability at cell freezing (%)
97
Antibiotics Used
free
Passage Number
P43
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
D5S818:12,13 D13S317:8 D7S820:8,10 D16S539:9 VWA:14 TH01:6 AM:X TPOX:8,11 CSF1PO:10,12
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - RPMI 1640
CO2 Conc.
5%
Viability immediately after thawing (%)
97
Additional information