JCRB0540 TIG-119

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0540 Cell Name   TIG-119
Profile   Normal human diploid cell from skin. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   6-year-old
Identity   available Tissue for Primary Cancer   skin
Case history   normal Metastasis   
Tissue Metastasized    Genetics   2N=46, XY
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   Maximum PD = 48. Cells may be distributed around PD15 - PD20.
Classify   normal Established by   Tokyo Metropolitan Institute of Gerontrogy (TMIG)
Registered by   TMIG Regulation for Distribution   
Comment    Year   2003
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Wash in CMF-PBS.Add 0.25% trypsin.Incubate at 37C for 10-15min.Suspend cells in medium.
Cell Number on Passage   5 x 10^4 cells/sq.cm. Race   Japanese
CO2 Conc.    Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3201
Reference
Pubmed id:7556460 Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.
Kondo H,Yonezawa Y
Exp Cell Res. 1995 Oct;220(2):501-4

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LOT Information

Cell No.   JCRB0540 Cell Name   
LOT No.   10162009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   1.8 - 2.5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 29 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   20
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0540 Cell Name   TIG-119
LOT No.   06052013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. (FBS; GIBCO Cat. #10099). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2.6 - 4.7 x 10^4 cells/sq.cm. Methods for Passages   Wash in CMF-PBS.Add 0.25% trypsin.Incubate at 37C for 10-15min.Suspend cells in medium.
Doubling Time   53 - 65 hr Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   77.8 Antibiotics Used   free
Passage Number   Unknown (4 at bank) PDL   30.1
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   77.8
Additional information   
Cell No.   JCRB0540 Cell Name   TIG-119
LOT No.   09022016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   1.5 - 4.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 23 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   PDL = 17.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.0
Additional information   
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