JCRB0540 TIG-119
Cell information
Important Notice(s)Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0540 | Cell Name | TIG-119 |
|---|---|---|---|
| Profile | Normal human diploid cell from skin. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 6-year-old |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | 2N=46, XY | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Maximum PD = 48. Cells may be distributed around PD15 - PD20. |
| Classify | normal | Established by | Tokyo Metropolitan Institute of Gerontrogy (TMIG) |
| Registered by | TMIG | Regulation for Distribution | |
| Comment | Year | 2003 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum. | Methods for Passages | Wash in CMF-PBS.Add 0.25% trypsin.Incubate at 37C for 10-15min.Suspend cells in medium. |
| Cell Number on Passage | 5 x 10^4 cells/sq.cm. | Race | Japanese |
| CO2 Conc. | Tissue Sampling | skin | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:7556460 | Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration. Kondo H,Yonezawa Y Exp Cell Res. 1995 Oct;220(2):501-4 |
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LOT Information
| Cell No. | JCRB0540 | Cell Name | |
|---|---|---|---|
| LOT No. | 10162009 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099). This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | 1.8 - 2.5 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | approx. 29 hrs | Cell Number in Vial (cells/1ml) | 1.1 x 10^6 |
| Viability at cell freezing (%) | 98.1 | Antibiotics Used | free |
| Passage Number | Unknown (3 at bank) | PDL | 20 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0540 | Cell Name | TIG-119 |
|---|---|---|---|
| LOT No. | 06052013 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum. (FBS; GIBCO Cat. #10099). This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | 2.6 - 4.7 x 10^4 cells/sq.cm. | Methods for Passages | Wash in CMF-PBS.Add 0.25% trypsin.Incubate at 37C for 10-15min.Suspend cells in medium. |
| Doubling Time | 53 - 65 hr | Cell Number in Vial (cells/1ml) | 1.7 x 10^6 |
| Viability at cell freezing (%) | 77.8 | Antibiotics Used | free |
| Passage Number | Unknown (4 at bank) | PDL | 30.1 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 77.8 | Additional information |
| Cell No. | JCRB0540 | Cell Name | TIG-119 |
|---|---|---|---|
| LOT No. | 09022016 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | 1.5 - 4.7 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 23 hrs. | Cell Number in Vial (cells/1ml) | 1.1 x 10^6 |
| Viability at cell freezing (%) | 89.0 | Antibiotics Used | free |
| Passage Number | Unknown (3 at bank) | PDL | PDL = 17.7 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 89.0 | Additional information |