JCRB0099 HH

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0099 Cell Name   HH
Profile   carotid artery normal endothelial cell Other Name   HH
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex    Age   
Identity    Tissue for Primary Cancer   vascular system
Case history   normal Metastasis   
Tissue Metastasized    Genetics   carotid artery endothelium, primary
Life Span   finite Crisis PDL   60-70 pas.
Morphology   epithelial-like Character   carotid artery endothelium, primary
Classify   normal Established by   Hagiwara,H. & Inada,Y.
Registered by   Inada,Y. Regulation for Distribution   No
Comment   Do not culture over passage 25. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.05 % trypsin.
Cell Number on Passage   8x10^5 cells/100mm dish Race   
CO2 Conc.   5 % Tissue Sampling   carotid artery
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2942
Reference
Pubmed id:6538703Sitosterol-stimulative production of plasminogen activator in cultured endothelial cells from bovine carotid artery.
Hagiwara H,Shimonaka M,Morisaki M,Ikekawa N,Inada Y
Thromb Res. 1984 Feb 15;33(4):363-70

Images



LOT Information

Cell No.   JCRB0099 Cell Name   HH
LOT No.   03082013 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   0.4-2x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA.Split ratio=1/4.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.88x10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   p17 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0099 Cell Name   
LOT No.   05232000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca 26～35 hrs Cell Number in Vial (cells/1ml)   9.4 x 10^5
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   P13 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as bovine by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS-EMEM CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0099 Cell Name   HH
LOT No.   121495 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (Cell Culture Lab.00939-1) Temperature   37 C
Cell Density at Seeding   1.3 x 10^5 cells/ml Methods for Passages   Wash cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5 min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.9x10^6
Viability at cell freezing (%)   90.1 Antibiotics Used   free
Passage Number   P14 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH, G6PD, NP bovine confirmed
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0099 Cell Name   HH
LOT No.   022693 Lot Specification   distribution
Medium   Eagle's minimal essential medium with Heparin-Na(100 ug/ml) and 10% FBS (endothelial growth suppl.) Temperature   37 C
Cell Density at Seeding   8x10^5 cells/10cm dish Methods for Passages   Treat twice with 0.25% trypsin-0.02% EDTA. 7% CO2 is recommended. Collagen or gelatin coated dish.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   P17 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0099 Cell Name   HH
LOT No.   070488 Lot Specification   distribution
Medium   MEM + FCS 10% Temperature   37
Cell Density at Seeding   8x10^5 cells/10cm dish Methods for Passages   0.02% EDTA and 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0099 Cell Name   
LOT No.   10222007 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37C
Cell Density at Seeding   3.0 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS for 5min at 37C
Doubling Time   approx. 40hr Cell Number in Vial (cells/1ml)   9.5 x 10^5
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as bovine by NP, G6PD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0099 Cell Name   HH
LOT No.   10292013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FBS; GIBCO Cat. # 10099). Temperature   37C
Cell Density at Seeding   0.4 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25%trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   4.8 x 10^6 (cells/1
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined. Bovine confirmed.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   94.5 Additional information   
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