-Detailed Information [JCRB0801]-

JCRB0801 Caki-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0801	Cell Name	Caki-1
Profile	renal clear cell carcinoma	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	49
Identity	available	Tissue for Primary Cancer	kidney
Case history		Metastasis
Tissue Metastasized		Genetics	renal cancer
Life Span		Crisis PDL
Morphology		Character	renal cancer
Classify		Established by	Fogh, J.
Registered by	Akiyama, M.	Regulation for Distribution
Comment		Year	1986
Medium	Eagle's minimal essential medium with 10% fetal calf serum.	Methods for Passages	Treat cells with 0.25% trypsin & dilute in fresh medium.Change medium twice a week.
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	renal
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0234
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	905963

Reference
Pubmed id:327080	One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. Fogh J,Fogh JM,Orfeo T J Natl Cancer Inst. 1977 Jul;59(1):221-6
Pubmed id:833871	Absence of HeLa cell contamination in 169 cell lines derived from human tumors. Fogh J,Wright WC,Loveless JD J Natl Cancer Inst. 1977 Feb;58(2):209-14

Images

LOT Information

07142008
03262008
092195
092298
03132015
07032020
12222023

Cell No.	JCRB0801	Cell Name
LOT No.	07142008	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	approx. 4 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA. Subculture twice every week at split ratio 1:4.
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	1.4 x 10^6
Viability at cell freezing (%)	80.4	Antibiotics Used	free
Passage Number	P54	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0801	Cell Name	Caki-1
LOT No.	03262008	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(Intergen RB52305)	Temperature	37 C
Cell Density at Seeding	1.3x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.5x10^6
Viability at cell freezing (%)	99.6	Antibiotics Used	NT
Passage Number	p52	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0801	Cell Name
LOT No.	092195	Lot Specification	distribution
Medium	MEM + 10% FCS	Temperature	37
Cell Density at Seeding	split ratio=1:6	Methods for Passages	0.25% trypsin. This cell line grows very slowly. Change medium twice a week.
Doubling Time	about 1 week	Cell Number in Vial (cells/1ml)	ca3-5x10^6
Viability at cell freezing (%)		Antibiotics Used	penicillin/streptmycin
Passage Number	P40	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	confirmed as human
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0801	Cell Name
LOT No.	092298	Lot Specification	distribution
Medium	Eagle's minimal essentiatl medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121)	Temperature	37 C
Cell Density at Seeding	5-7 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	65-70 hrs	Cell Number in Vial (cells/1ml)	4.2 x 10^5
Viability at cell freezing (%)	80.0	Antibiotics Used	free
Passage Number	P53	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0801	Cell Name	Caki-1
LOT No.	03132015	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	1.0 - 4.5 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 28 hrs	Cell Number in Vial (cells/1ml)	1.2 x 10^6
Viability at cell freezing (%)	87.6	Antibiotics Used	free
Passage Number	P53	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:11,12 D13S317:11,12 D7S820:8,12 D16S539:12 VWA:15,17 TH01:6,8 AM:X TPOX:8,11 CSF1PO:10,11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	87.6	Additional information

Cell No.	JCRB0801	Cell Name	Caki-1
LOT No.	07032020	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	1.9 - 3.1 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 42 hrs.	Cell Number in Vial (cells/1ml)	7.4 x 10^5
Viability at cell freezing (%)	99	Antibiotics Used	free
Passage Number	P55	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	99	Additional information

Cell No.	JCRB0801	Cell Name	Caki-1
LOT No.	12222023	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS; NICHIREI Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	2.0 - 3.6 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	22 - 65 hrs	Cell Number in Vial (cells/1ml)	1.5 x 10^6
Viability at cell freezing (%)	100	Antibiotics Used	free
Passage Number	P55	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	74.4	Additional information