-Detailed Information [JCRB0106]-

JCRB0106 SCCH-26

Cell information

Important Notice(s)

ES medium was developed by Koyama,H. and Kodama,H. (Cancer Res., 42: 4210-4214, 1982).

The composition of ES medium is based on Eagle's minimum essential medium (E-MEM) plus seven non-essential amino acids (NEAA), pyruvate and cyanocobalamin.


Eagle's minimum essential medium: GIBCO (Thermofisher) cat. # 11095-080

Non essential amino acids: 0.2 mM each (final conc). 

Non-essential amino acids for Eagle's MEM is commercially available, such as,
non-essential amino acid solution (10 mM each), GIBCO (Thermofisher) Cat. No. 11140-050.
It should be diluted to 1:50 to E-MEM.

Sodium pyruvate: 1 mM (final conc)
Concentrated sodium pyruvate solution is available, such as,
Sodium pyruvate solution 100 mM (100X): GIBCO (Thermofisher) cat. # 11360-070
 
0.1 mg/L cyanocobalamin (vitamine B12))final conc)
Dissolve the powder to water to prepare 1000 X stock solution (10 mg/L), and filter sterilize.

The below is in Japanse.

ES培地は、Eagle's MEMに7種類の非必須アミノ酸、ピルビン酸ナトリウム、cyanocobalaminを加えた組成のものです（ES細胞培養用のES培地とは異なります）。市販品は以前は日水製薬(株)が品番05971で販売しておりましたが、現在カタログ落ちしているようです。

次のように調製してください。

Eagle's MEM： Thermofisher (GIBCO) 品番11095-080　(500 mLの液体培地です。

非必須アミノ酸溶液は、例えば下記のような100X 溶液がGIBCOから販売されています。
MEM non-essential amino acids solution 10 mM (100X): Thermofisher (GIBCO) 品番11140-050
しかし、ES培地の非必須アミノ酸濃度は0.2 mMであるため、50Xに希釈してください。

ピルビン酸ナトリウムは、100X溶液がGIBCOから販売されています。
Sodium pyruvate solution 100 mM (100X) ： Thermofisher (GIBCO) 品番11360-070

cyanocobalamin (vitamin B12)は粉末単品として、富士フィルム和光純薬などから購入し、
1000倍ストック水溶液を作って濾過滅菌、培地に添加してください。
培地中での最終濃度は 0.1 mg/L cyanocobalamin (vitamine B12)です。 



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Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0106	Cell Name	SCCH-26
Profile	Human neuroblastoma cell line.	Other Name	(eliminated)
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	neural
Case history	neuroblastoma	Metastasis	No
Tissue Metastasized		Genetics	neuroblastoma
Life Span	infinite	Crisis PDL
Morphology	fibroblast-like	Character	neuroblastoma
Classify	tumor	Established by	Kaneko,Y.
Registered by	Kaneko,Y.	Regulation for Distribution	Not regulated
Comment	Other name was discarded because of the personal ID.	Year	1987
Medium	ES medium with 10% calf serum. The ES medium is Eagle's MEM (containing L-glutamine) supplemented with 0.2 mM each non essential amino acids, 1 mM sodium pyruvate, and 0.1 mg/L cyanocobalamin (vitamine B12).	Methods for Passages	Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage	split ratio=1/10	Race
CO2 Conc.	5 %	Tissue Sampling
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	+	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes	EBV(low copy)

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1686

Reference
Pubmed id:2121721	Monoclonal antibodies to disialogangliosides: characterization of antibody-mediated cytotoxicity against human melanoma and neuroblastoma cells in vitro. Kawashima I,Tada N,Fujimori T,Tai T J Biochem. 1990 Jul;108(1):109-15
Pubmed id:7105014	Adenine phosphoribosyltransferase deficiency in cultured mouse mammary tumor FM3A cells resistant to 4-carbamoylimidazolium 5-olate. Koyama H,Kodama H Cancer Res. 1982 Oct;42(10):4210-4

Images

LOT Information

Cell No.	JCRB0106	Cell Name	SCCH-26
LOT No.	101795	Lot Specification	distribution
Medium	ES medium with 10% fetal bovine serum (JRHB B1B1062)	Temperature	37 C
Cell Density at Seeding	4.0x10^5 cells/sq.cm.	Methods for Passages	Mechanical dispersion by pipetting
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.07x10^6
Viability at cell freezing (%)		Antibiotics Used	Kanamycine 60ug/ml
Passage Number	P9*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% dimethyl sulfoxide (DMSO)	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0106	Cell Name	SCCH-26
LOT No.	033092	Lot Specification	distribution
Medium	ES medium with 10% fetal calf serum	Temperature	37 C
Cell Density at Seeding	1x10^6 cells/ml	Methods for Passages	0.02 % EDTA + 0.25 % trypsin
Doubling Time		Cell Number in Vial (cells/1ml)	3.6x10^6
Viability at cell freezing (%)	68.2	Antibiotics Used	free
Passage Number	P5*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	Culture medium with 5% DMSO	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0106	Cell Name	SCCH-26
LOT No.	102590	Lot Specification	distribution
Medium	ES + FCS 10%	Temperature	37
Cell Density at Seeding	split ratio:1/10	Methods for Passages	0.02% EDTA and 0.25% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.0x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P7*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	NT
Sterility: FUNGI	NT	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0106	Cell Name	SCCH-26
LOT No.	111987	Lot Specification	distribution
Medium	ES + FCS 10%	Temperature	37
Cell Density at Seeding	split ratio:1/10	Methods for Passages	0.02% EDTA and 0.25% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	8x10^5
Viability at cell freezing (%)	65%	Antibiotics Used	KM
Passage Number	P4*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD,LD,NP
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0106	Cell Name
LOT No.	03082000	Lot Specification	distribution
Medium	ES medium (Nissui Seiyaku) with 10% calf serum	Temperature	37 C
Cell Density at Seeding	6-14 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA. Growth retards at high cell density. Passage at 30-50% confluency.
Doubling Time	3 days	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	53	Antibiotics Used	free
Passage Number	P7	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

JCRB Cell Bank

JCRB0106 SCCH-26

Cell information

LOT Information