JCRB0110 M1-T22
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0110 | Cell Name | M1-T22 |
|---|---|---|---|
| Profile | Spontaneously occured myeloid leukemia cell line differentiated by dexamethasone or etc. | Other Name | M1 clone T22 |
| Animal | mouse | Strain | SL |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | spontaneous myeloid leukemia | Metastasis | |
| Tissue Metastasized | Genetics | cytologically determined as myeloblast | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Cells are differentiated by dexamethasone or etc. |
| Classify | tumor | Established by | Ichikawa,Y. |
| Registered by | Hozumi,M. | Regulation for Distribution | Not regulated. |
| Comment | Year | 1987 | |
| Medium | Eagle's minimal essential medium with x2 amino acids-vitamins and 10% fetal calf serum | Methods for Passages | Dilute every 3 days. |
| Cell Number on Passage | 1-2x10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:4517230 | Spontaneous development of macrophage-like cells in a culture of myeloid leukemia cells. Maeda M,Ichikawa Y Gan. 1973 Jun;64(3):265-71 |
| Pubmed id:4187945 | Differentiation of a cell line of myeloid leukemia. Ichikawa Y J Cell Physiol. 1969 Dec;74(3):223-34 |
LOT Information
| Cell No. | JCRB0110 | Cell Name | M1-T22 |
|---|---|---|---|
| LOT No. | 051592 | Lot Specification | distribution |
| Medium | MEM + AA with vitamin (lot#.041592) + FBS 10% (lot. Cell Cult.Labs.00939-01) | Temperature | 37 C |
| Cell Density at Seeding | 1-2x10^6 cells/ml | Methods for Passages | dilution (dilute to 1-2x10^5 cells/ml with 3-4 days interval). Minimum inoculum size=2x10^4cells/ml. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.7x10^6 |
| Viability at cell freezing (%) | 94.3 | Antibiotics Used | free |
| Passage Number | P15* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0110 | Cell Name | M1-T22 |
|---|---|---|---|
| LOT No. | 110788 | Lot Specification | distribution |
| Medium | MEM w/ x2 amino acids, vitamins + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 1-2x10^5 cells/ml | Methods for Passages | Dilute every 3 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.7x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P14* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |