JCRB0111 VERO

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0111 Cell Name   VERO
Profile   African Green Monkey kidney normal cell line. Other Name   Vero
Animal   monkey, African green Strain   African green
Genus   Cercopithecus Species   aethiops
Sex    Age   
Identity   available Tissue for Primary Cancer   kidney
Case history   normal Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Normal cell with contact inhibition
Classify   transformed Established by   Yasumura,Y. & Kawakita
Registered by   Simizu,B. Regulation for Distribution   No regulation applied.
Comment   Cells submitted have lower passage number than the cells kept in ATCC. Year   1987
Medium   Eagle's minimal essential medium with 5% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/2 - 1/6 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0059
Reference
Pubmed id:35391802Whole-Genome Sequencing of Vero E6 (VERO C1008) and Comparative Analysis of Four Vero Cell Sublines.
Konishi K,Yamaji T,Sakuma C,Kasai F,Endo T,Kohara A,Hanada K,Osada N
Front Genet. 2022;13():801382
Pubmed id:15234806Comparative study of influenza virus replication in Vero and MDCK cell lines.
Youil R,Su Q,Toner TJ,Szymkowiak C,Kwan WS,Rubin B,Petrukhin L,Kiseleva I,Shaw AR,DiStefano D
J Virol Methods. 2004 Sep 1;120(1):23-31
Pubmed id:15938255A plaque neutralization method for arboviruses.
Earley E,Peralta PH,Johnson KM
Proc Soc Exp Biol Med. 1967 Jul;125(3):741-7
Pubmed id:4961494Replication of rubella virus in a continuous line of African green monkey kidney cells (Vero).
Liebhaber H,Riordan JT,Horstmann DM
Proc Soc Exp Biol Med. 1967 Jun;125(2):636-43
Pubmed id:5591575Growth of Junin virus, the etiological agent of Argentinian hemorrhagic fever, in cell cultures.
Rhim JS,Simizu B,Wiebenga NH
Arch Gesamte Virusforsch. 1967;21(2):243-52
Pubmed id:5833688Studies on measles virus. II. Propagation in two established simian renal cell lines and development of a plaque assay.
Sasaki K,Makino S,Kasahara S
Kitasato Arch Exp Med. 1964 Dec;37(1):27-42


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Pubmed id:25267831 The genome landscape of the african green monkey kidney-derived vero cell line.
Osada N,Kohara A,Yamaji T,Hirayama N,Kasai F,Sekizuka T,Kuroda M,Hanada K
DNA Res. 2014 Dec;21(6):673-83

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LOT Information

Cell No.   JCRB0111 Cell Name   VERO
LOT No.   12182012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS, GIBCO Cat.# 10099) Temperature   37 C
Cell Density at Seeding   5.2 - 7.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   87.6 Antibiotics Used   free
Passage Number   P116 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0111 Cell Name   
LOT No.   11292007 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 5% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   3 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 18 hrs Cell Number in Vial (cells/1ml)   4.0 x 10^6
Viability at cell freezing (%)   90.5 Antibiotics Used   free
Passage Number   P116 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as African green monkey by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0111 Cell Name   VERO
LOT No.   072793 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 5% fetal bovine serum (J.R.H.Biosciences 1B1062) Temperature   37 C
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   87.2 Antibiotics Used   free
Passage Number   P118 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0111 Cell Name   VERO
LOT No.   092990 Lot Specification   distribution
Medium   MEM + FCS 5 % Temperature   37
Cell Density at Seeding   split ratio:1/2 - 1/6 Methods for Passages   0.02 % EDTA and 0.25 % trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P113 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   G6PD, LD, NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0111 Cell Name   VERO
LOT No.   061788 Lot Specification   distribution
Medium   MEM + FCS 5 % Temperature   37
Cell Density at Seeding   split ratio:1/2 - 1/6 Methods for Passages   0.02 % EDTA and 0.25 % trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8X10^6
Viability at cell freezing (%)   94% Antibiotics Used   free
Passage Number   P114 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   PEPB,NP,LD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0111 Cell Name   VERO
LOT No.   11022016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 5% fetal bovine serum (FBS; Sigma Cat.# 172012) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   P116 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
Cell No.   JCRB0111 Cell Name   VERO
LOT No.   11062018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 5% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   5.0 - 9.15 x 10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.55 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P117 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
Images
Cell No.   JCRB0111 Cell Name   VERO
LOT No.   03272024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 5% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P117 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
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