JCRB0113 PAI

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0113 Cell Name   PAI
Profile   myeloma, (BALB/c mouse) Other Name   
Animal   mouse Strain   BALB/C
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   myeloma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   non screeting type for IgE.
Classify   transformed Established by   Stocker,J.W. et al.
Registered by   Arai, T. Regulation for Distribution   
Comment    Year   1987
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution by gentle pipetting.
Cell Number on Passage   10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J288
Reference
Pubmed id:1703558Analysis of the newly identified neutralization epitopes on VP7 of human rotavirus serotype 1.
Kobayashi N,Taniguchi K,Urasawa S
J Gen Virol. 1991 Jan;72 ( Pt 1)():117-24
Pubmed id:1701477Identification of operationally overlapping and independent cross-reactive neutralization regions on human rotavirus VP4.
Kobayashi N,Taniguchi K,Urasawa S
J Gen Virol. 1990 Nov;71 ( Pt 11)():2615-23
Pubmed id:noneGeneration of 2 new mouse myeloma cell lines ’PAI’ and ’PAI-0’ for hybridoma production.
Stocker, J.W., Forster, H.K., Miggiano, M., Stahli, C., Staiger, G., Takacs, B., and Staehelin, T.
Res. Disclos., 217: 155-157, 1982.




LOT Information

Cell No.   JCRB0113 Cell Name   
LOT No.   05172000 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   ca 19 hrs Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as moue by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium containing 10% DMSO and 20% FCS CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0113 Cell Name   PAI
LOT No.   061191 Lot Specification   distribution
Medium   RPMI1640 + FCS 10 % Temperature   37 C
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Dilution by gentle pipetting.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0113 Cell Name   PAI
LOT No.   101988 Lot Specification   distribution
Medium   RPMI1640 + FCS 10 % Temperature   37
Cell Density at Seeding   10^5 cells/ml Methods for Passages   Dilution by gentle pipetting.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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