JCRB0113 PAI
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0113 | Cell Name | PAI |
|---|---|---|---|
| Profile | myeloma, (BALB/c mouse) | Other Name | |
| Animal | mouse | Strain | BALB/C |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | myeloma | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | non screeting type for IgE. |
| Classify | transformed | Established by | Stocker,J.W. et al. |
| Registered by | Arai, T. | Regulation for Distribution | |
| Comment | Year | 1987 | |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | Dilution by gentle pipetting. |
| Cell Number on Passage | 10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1703558 | Analysis of the newly identified neutralization epitopes on VP7 of human rotavirus serotype 1. Kobayashi N,Taniguchi K,Urasawa S J Gen Virol. 1991 Jan;72 ( Pt 1)():117-24 |
| Pubmed id:1701477 | Identification of operationally overlapping and independent cross-reactive neutralization regions on human rotavirus VP4. Kobayashi N,Taniguchi K,Urasawa S J Gen Virol. 1990 Nov;71 ( Pt 11)():2615-23 |
| Pubmed id:none | Generation of 2 new mouse myeloma cell lines ’PAI’ and ’PAI-0’ for hybridoma production. Stocker, J.W., Forster, H.K., Miggiano, M., Stahli, C., Staiger, G., Takacs, B., and Staehelin, T. Res. Disclos., 217: 155-157, 1982. |
LOT Information
| Cell No. | JCRB0113 | Cell Name | |
|---|---|---|---|
| LOT No. | 05172000 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal calf serum | Temperature | 37 C |
| Cell Density at Seeding | 1 x 10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | ca 19 hrs | Cell Number in Vial (cells/1ml) | 3.0 x 10^6 |
| Viability at cell freezing (%) | 87 | Antibiotics Used | free |
| Passage Number | P7* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as moue by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | Culture medium containing 10% DMSO and 20% FCS | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0113 | Cell Name | PAI |
|---|---|---|---|
| LOT No. | 061191 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 10 % | Temperature | 37 C |
| Cell Density at Seeding | 10^5 cells/ml | Methods for Passages | Dilution by gentle pipetting. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.0x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P8* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0113 | Cell Name | PAI |
|---|---|---|---|
| LOT No. | 101988 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 10 % | Temperature | 37 |
| Cell Density at Seeding | 10^5 cells/ml | Methods for Passages | Dilution by gentle pipetting. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.0x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P5* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | LD |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0113 | Cell Name | PAI |
|---|---|---|---|
| LOT No. | 07252024 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 1.3 - 1.8 x 10^5 cells/mL | Methods for Passages | Cells were harvested by gentle pipetting, and the cells were simply diluted to fresh medium. |
| Doubling Time | approx. 1 day (at log phase) | Cell Number in Vial (cells/1ml) | 6.3 x 10^5 |
| Viability at cell freezing (%) | 71.1 | Antibiotics Used | free |
| Passage Number | Unknown (7 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Y/N | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 84.0 | Additional information |