JCRB0202 B16 melanoma

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0202 Cell Name   B16 melanoma
Profile   Mouse skin melanoma cell line. Other Name   B16
Animal   mouse Strain   C57BL/6
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   melanoma
Case history   melanoma Metastasis   
Tissue Metastasized    Genetics   melanoma
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   positive for melanin pigment production but large portion of cells are amelanotic
Classify   tumor Established by   Sillagi,S.
Registered by   Isaka,H. Regulation for Distribution   free
Comment   Melanin producing tumor cell line. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25 % trypsin.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   skin (melanoma)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_F936
Reference
Pubmed id:4981070 Control of pigment production in mouse melanoma cells in vitro. Evocation and maintenance.
Silagi S
J Cell Biol. 1969 Nov;43(2):263-74
Pubmed id:14234007 THE ISOLATION AND CYTOLOGY OF TWO PIGMENT CELL STRAINS FROM B-16 MOUSE MELANOMAS.
HU F,LESNEY PF
Cancer Res. 1964 Oct;24():1634-43
Pubmed id:13973915 Enzymatic determination of transmissible replicating factors associated with mouse tumors.
RILEY V
Ann N Y Acad Sci. 1963 Feb 15;100():762-90

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LOT Information

Cell No.   JCRB0202 Cell Name   
LOT No.   03232009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 1.8 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 20 hrs Cell Number in Vial (cells/1ml)   5.1 x 10^6
Viability at cell freezing (%)   98.8 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0202 Cell Name   
LOT No.   10062005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   3 - 7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   95.8 Antibiotics Used   free
Passage Number   Unknown (P5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0202 Cell Name   
LOT No.   06122003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   <10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = 20 - 21 hrs Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   Unknown(5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0202 Cell Name   B16 melanoma
LOT No.   021696 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (Flow Labs.0070893) Temperature   37 C
Cell Density at Seeding   5.1x10^5 cells/ml Methods for Passages   Cells washed once with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 3 min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.5x10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH, G6PD, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0202 Cell Name   
LOT No.   032993 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37
Cell Density at Seeding   2x10^4/ml Methods for Passages   0.25 % trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   4.0 x 10^5
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0202 Cell Name   
LOT No.   07242000 Lot Specification   distribution
Medium   Eagle's minimal esseintial medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.= ca 28 hrs Cell Number in Vial (cells/1ml)   8.8 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0202 Cell Name   B16 melanoma
LOT No.   11102013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FBS; GIBCO Cat. # 10099). Temperature   37C
Cell Density at Seeding   1.4 - 1.6 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25%trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   9.9 x 10^6 (cells/1
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined. Mouse confirmed.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   97.6
Additional information   Lot# 11192013 is mislabel. Correctly: 11102013.
Cell No.   JCRB0202 Cell Name   B16 melanoma
LOT No.   03312017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   2.6 - 4.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96.2
Additional information   
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