Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0206 Cell Name   PCC4 AG
Profile   teratocarcinoma, 8-azaguanine-resistant, (129/Sv mouse) Other Name   
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   teratoma
Case history    Metastasis   
Tissue Metastasized    Genetics   testis, AG-r
Life Span   infinite Crisis PDL   
Morphology    Character   testis, AG-r. Cells originally contaminated with mycoplasmas but decotanimated by MC210 in the JCRB cell bank.
Classify    Established by   Jacob,H.
Registered by   Isaka,H. Regulation for Distribution   Report to Dr.Sekiguchi is required.
Comment   Resistant to 8-azaguanine. Year   1985
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   cybrid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5855
Pubmed id:6813981Induction of supermelanin synthesis and morphological changes in interspecific reconstituted cells and its reversal by tumor promoter.
Sekiguchi T,Tosu M,Yoshida MC,Oikawa A,Ishihara K,Fujiki H,Tumuraya M,Kameya T
Somatic Cell Genet. 1982 Sep;8(5):605-22
Pubmed id:6931103Characterization of a human ovarian teratocarcinoma-derived cell line.
Zeuthen J,Nørgaard JO,Avner P,Fellous M,Wartiovaara J,Vaheri A,Rosén A,Giovanella BC
Int J Cancer. 1980 Jan 15;25(1):19-32
Pubmed id:510422Stimulation of differentiation of several murine embryonal carcinoma cell lines by retinoic acid.
Jetten AM,Jetten ME,Sherman MI
Exp Cell Res. 1979 Dec;124(2):381-91
Pubmed id:61803Cell lines derived from teratocarcinomas.
Nicolas JF,Avner P,Gaillard J,Guenet JL,Jakob H,Jacob F
Cancer Res. 1976 Nov;36(11 Pt. 2):4224-31
Pubmed id:4599372[Teratocarcinoma of the mouse: isolation, culture and properties of pluripotential cells].
Jakob H,Boon T,Gaillard J,Nicolas J,Jacob F
Ann Microbiol (Paris). 1973 Oct;124(3):269-82


LOT Information

Cell No.   JCRB0206 Cell Name   
LOT No.   022192 Lot Specification   distribution
Medium   DME: 10% FCS Temperature   37
Cell Density at Seeding   2x10^4 cells/ml Methods for Passages   0.02 % EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information