JCRB1053 STC 1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1053 Cell Name   STC 1
Profile   Human small cell lung carcinoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   tumor Established by   Nozawa,S. & Chou,T.
Registered by   Nozawa,S. & Chou,T. Regulation for Distribution   
Comment    Year   2003
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.025 % trypsin.
Cell Number on Passage   0.5-1x10^6 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   small cell carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3171
Reference
Pubmed id:1484432 [Generation of effector cells from primary lung cancer patients by stimulation with anti-CD3 monoclonal antibody followed by culture with recombinant interleukin-2].
Mizusawa A
Nihon Kyobu Shikkan Gakkai Zasshi. 1992 Nov;30(11):1937-45
Pubmed id:6265940 Selective growth in serum-free hormone-supplemented medium of tumor cells obtained by biopsy from patients with small cell carcinoma of the lung.
Carney DN,Bunn PA Jr,Gazdar AF,Pagan JA,Minna JD
Proc Natl Acad Sci U S A. 1981 May;78(5):3185-9
Pubmed id:none Establishment and Characterization of Multiple Drug-resistant Lung Cancer Cell Lines and Their Susceptability to Autologous Effector Lymphocytes
Nozawa, S.
Haigan, 32(2):223-232,1992

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LOT Information

Cell No.   JCRB1053 Cell Name   
LOT No.   04212010 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.7 - 1.3 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage once or twice a week.
Doubling Time   approx. 50 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   77.0 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1053 Cell Name   STC 1
LOT No.   06022003 Lot Specification   distribution
Medium   RPMI1640 medium with 10 % fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   2.3 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.025% trypsin and 0.02% EDTA. Subculture every 3-4 days and medium change every 2 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   87.0 Antibiotics Used   free
Passage Number   P20 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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