JCRB1053 STC 1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1053 | Cell Name | STC 1 |
|---|---|---|---|
| Profile | Human small cell lung carcinoma. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | lung cancer | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | tumor | Established by | Nozawa,S. & Chou,T. |
| Registered by | Nozawa,S. & Chou,T. | Regulation for Distribution | |
| Comment | Year | 2003 | |
| Medium | RPMI1640 medium with 10% fetal calf serum. | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.025 % trypsin. |
| Cell Number on Passage | 0.5-1x10^6 cells/60mm dish | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type | small cell carcinoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1484432 | [Generation of effector cells from primary lung cancer patients by stimulation with anti-CD3 monoclonal antibody followed by culture with recombinant interleukin-2]. Mizusawa A Nihon Kyobu Shikkan Gakkai Zasshi. 1992 Nov;30(11):1937-45 |
| Pubmed id:6265940 | Selective growth in serum-free hormone-supplemented medium of tumor cells obtained by biopsy from patients with small cell carcinoma of the lung. Carney DN,Bunn PA Jr,Gazdar AF,Pagan JA,Minna JD Proc Natl Acad Sci U S A. 1981 May;78(5):3185-9 |
| Pubmed id:none | Establishment and Characterization of Multiple Drug-resistant Lung Cancer Cell Lines and Their Susceptability to Autologous Effector Lymphocytes Nozawa, S. Haigan, 32(2):223-232,1992 |
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LOT Information
| Cell No. | JCRB1053 | Cell Name | |
|---|---|---|---|
| LOT No. | 04212010 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 0.7 - 1.3 x 10^5 cells/ml | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage once or twice a week. |
| Doubling Time | approx. 50 hrs | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 |
| Viability at cell freezing (%) | 77.0 | Antibiotics Used | free |
| Passage Number | P21 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1053 | Cell Name | STC 1 |
|---|---|---|---|
| LOT No. | 06022003 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10 % fetal calf serum (Intergen RB52305). | Temperature | 37 C |
| Cell Density at Seeding | 2.3 x 10^4 cells/sq.cm. | Methods for Passages | Cells were harvested after treatment with 0.025% trypsin and 0.02% EDTA. Subculture every 3-4 days and medium change every 2 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.6 x 10^6 |
| Viability at cell freezing (%) | 87.0 | Antibiotics Used | free |
| Passage Number | P20 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1053 | Cell Name | STC 1 |
|---|---|---|---|
| LOT No. | 05202019 | Lot Specification | distribution |
| Medium | RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 1.1 - 1.9 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.025% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 18 hrs. | Cell Number in Vial (cells/1ml) | 1.9 x 10^6 cells/mL |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | P22 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 76.2 | Additional information |