JCRB1063 KYSE140

Cell information

Important Notice(s)

KYSEシリーズ細胞株の民間企業への分譲について

On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)

KYSEシリーズ細胞株の使用について

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1063 Cell Name   KYSE140
Profile   Human esophageal cancer cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   54
Identity   available Tissue for Primary Cancer   esophagus
Case history   esophageal cancer Metastasis   
Tissue Metastasized    Genetics   modal chromosome no.=118XX, p53 mutation in exon 6, codon 193 (CAT->CGT).
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   tumor Established by   Shimada,Y. et.al.
Registered by   Shimada,Y. Regulation for Distribution   Cite reference {5368}. Company users require MTA from the originator. See additional information.
Comment   Distribution restricted to Japan(domestic use only). Year   2003
Medium   F12 medium with 2% fetal calf serum. Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   esophagus
Tissue Type   moderately differentiated squamous cell carci

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1347
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LOT Information

Cell No.   JCRB1063 Cell Name   
LOT No.   09042006 Lot Specification   distribution
Medium   Ham's F12 medium with 2% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   1.2 - 1.8 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   92.5 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1063 Cell Name   KYSE140
LOT No.   01292016 Lot Specification   distribution2
Medium   Ham's F12 medium(SIGMA) with 5% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.39-3.13x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.72x10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free
Passage Number   p14* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:12
D7S820:10,11
D16S539:10,12
VWA:14
TH01:7,9
AM:X
TPOX:8
CSF1PO:13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   98
Additional information   Mycoplasma and Acholeplasma has eliminated with Plasmocin(Final concentration 25 ug/ml).
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Cell No.   JCRB1063 Cell Name   KYSE140
LOT No.   12102015 Lot Specification   distribution
Medium   Ham's F12 medium(SIGMA) with 5% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.0-1.87x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.51x10^6
Viability at cell freezing (%)   92.00 Antibiotics Used   free
Passage Number   p12* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:12
D7S820:10,11
D16S539:10,12
VWA:14
TH01:7,9
AM:X
TPOX:8
CSF1PO:13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   79
Additional information   Mycoplasma and Acholeplasma has eliminated with Plasmocin(Final concentration 25 ug/ml).
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