JCRB1066 KMH-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1066 Cell Name   KMH-2
Profile   Human anaplasitc thyroid carcinoma cell line with multidrug resistance, derived from a left cervical tumor with a sligt leukocytosis. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   71
Identity   available Tissue for Primary Cancer   thyroid
Case history   left cervical tumor with a slight leukocytosis, a biopsy revealed an anaplastic thyroid. Metastasis   
Tissue Metastasized    Genetics   Chromosome No. 92-108.
Life Span   infinite Crisis PDL   
Morphology   spindle shaped cells with giant cells Character   doubling time is 58 hours and multidrug registance.
Classify   tumor Established by   Sekiguchi,K. et. al.
Registered by   Sekiguchi,K. Regulation for Distribution   no
Comment    Year   2003
Medium   1 to 1 mixture of Dulbecco's modified Eagle's medium and RPMI1640 medium with 10% heat inactivated feta; bovine serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin.
Cell Number on Passage   10^4 cell/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   cervical tumor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_S641
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298167
Reference
Pubmed id:11686581Biological characteristics and chemosensitivity profile of four human anaplastic thyroid carcinoma cell lines.
Sekiguchi M,Shiroko Y,Arai T,Kishino T,Sugawara I,Kusakabe T,Suzuki T,Yamashita T,Obara T,Ito K,Hasumi K
Biomed Pharmacother. 2001 Oct;55(8):466-74

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LOT Information

Cell No.   JCRB1066 Cell Name   
LOT No.   01302004 Lot Specification   distribution
Medium   1 : 1 mixture of Dulbecco's modified Eagle's medium and RPMI1640 medium with 10% heat-inactivated fetal bovine serum (FBS Lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   4 - 10 x 10^4 cells/ml Methods for Passages   0.25% trypsin
Doubling Time   1.1 - 1.5 days Cell Number in Vial (cells/1ml)   7.4 x 10^5
Viability at cell freezing (%)   93.7 Antibiotics Used   free
Passage Number   P278 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% heat-inactivated FBS in DMEM:RPMI1640 = 1:1 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1066 Cell Name   KMH-2
LOT No.   04192022 Lot Specification   distribution
Medium   1 : 1 mixture of Dulbecco's modified Eagle's medium (GIBCO,Cat No.11885-084) and RPMI1640 medium ( GIBCO,Cat No.11875-093) with 10% heat-inactivated fetal bovine serum (Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   1.07.-6.61x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.41x10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   p279 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:9
D7S820:11
D16S539:9,12
VWA:14,15
TH01:9
AM:X,Y
TPOX:8,11
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   90.7 Additional information   
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