JCRB0212 B82

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0212 Cell Name   B82
Profile   skin fibroblast, bromodeoxyuridine-resistant Other Name   B82
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   skin
Case history   BrdU resistant Metastasis   
Tissue Metastasized    Genetics   skin fibroblast, BrdU-r
Life Span    Crisis PDL   
Morphology   fibroblast-like Character   fibroblast
Classify   transformed Established by   Littlefield,J.W.
Registered by   Isaka,H. Regulation for Distribution   
Comment   Resistant to BrdU. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   
Cell Number on Passage   2 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4114
Pubmed id:4952025The use of drug-resistant markers to study the hybridization of mouse fibroblasts.
Littlefield JW
Exp Cell Res. 1966 Jan;41(1):190-6


LOT Information

Cell No.   JCRB0212 Cell Name   B82
LOT No.   022098 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (Summit,lot#8.1+42). Temperature   37 C
Cell Density at Seeding   2x10^4 cells/sq.cm. Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA. Subculture every three days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.84x10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LD, NP were tested and confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information