JCRB1073 ASH-3

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1073 Cell Name   ASH-3
Profile   Tumor cell line with multidrug registance obtained from rapidly growing cervical tumor(neck) diagnosed as an anaplastic thyroid carcinoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   68
Identity   available Tissue for Primary Cancer   thyroid
Case history   a rapidly growint cervical tumor. Diagnosis was an anaplastic thyroid carcinoma made by a biopsy. Metastasis   
Tissue Metastasized    Genetics   Chromosome number was 79-89.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Doubling time is 84 hours and multidurg registance cell line.
Classify   tumor Established by   Sekiguchi,M. et. al.
Registered by   Sekiguchi,M. Regulation for Distribution   not applied
Comment   Cells were identified to be unique on 10/17/2001. Year   2003
Medium   1 to 1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 10% heat inactivated fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin.
Cell Number on Passage   10^5 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   cervical tissue
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2856
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1290722
Reference
Pubmed id:11686581 Biological characteristics and chemosensitivity profile of four human anaplastic thyroid carcinoma cell lines.
Sekiguchi M,Shiroko Y,Arai T,Kishino T,Sugawara I,Kusakabe T,Suzuki T,Yamashita T,Obara T,Ito K,Hasumi K
Biomed Pharmacother. 2001 Oct;55(8):466-74

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LOT Information

Cell No.   JCRB1073 Cell Name   ASH-3
LOT No.   10272003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium and RPMI1640 medium (1:1) with 10% heat inactivated bovine serum (RB52405). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   P224 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:12
D7S820:11
D16S539:9
VWA:16,17
TH01:9
AM:X
TPOX:8,11
CSF1PO:10,14
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1073 Cell Name   ASH-3
LOT No.   12072015 Lot Specification   distribution
Medium   1 to 1 mixture of Dulbecco's modified Eagle's medium and RPMI-1640 medium with 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   0.9 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   43 - 47 hrs Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   P226 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS DMEM:RPMI-1640=1:1 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94.2
Additional information   
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