-Detailed Information [JCRB1075.0]-

JCRB1075.0 IM95

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB1075.0	Cell Name	IM95
Profile	Human gastric cancer cell line.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	63
Identity	available	Tissue for Primary Cancer	stomach
Case history	moderately differentiated adenocarcinoma of stomach	Metastasis
Tissue Metastasized		Genetics	Modal chromosome number = 46.
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	Td=25 hrs. CEA related antigen observed. HGF production and tumorigenesity is maintained in the cell line.
Classify	tumor	Established by	Iwai,Y. & Matsuda,M.
Registered by	Iwai,M.	Regulation for Distribution
Comment		Year	2003
Medium	Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal bovine serum and 10mg/L insulin.	Methods for Passages	Cells were harvested after the treatment with 0.3% trypsin and 0.03% EDTA.
Cell Number on Passage		Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	stomach
Tissue Type	stomach cancer

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_2961
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1240155

Reference
Pubmed id:14609362	Cloning of a cancer cell-producing hepatocyte growth factor, vascular endothelial growth factor, and interleukin-8 from gastric cancer cells. Iwai M,Matsuda M,Iwai Y In Vitro Cell Dev Biol Anim. 2003 Jul-Aug;39(7):288-90

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LOT Information

10132009
11162005
09302016
03302021

Cell No.	JCRB1075.0	Cell Name
LOT No.	10132009	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium with 4.5 g/L glucose (high glucose) 10 ug/ml insulin and 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	3.3 - 4.6 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 46 hrs	Cell Number in Vial (cells/1ml)	4.3 x 10^6
Viability at cell freezing (%)	94.0	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:9,11 D13S317:10,12 D7S820:9.3,10.3 D16S539:9,10 VWA:17,18,19 TH01:6,10 AM:X TPOX:8,10 CSF1PO:11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM (high glucose)	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1075.0	Cell Name
LOT No.	11162005	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (high glucose; 4.5 g/L) with 10 ug/ml insulin and 10% fetal bovine serum (FBS lot; GIBCO 4218333S)	Temperature	37 C
Cell Density at Seeding	4 - 8 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 1.2 days	Cell Number in Vial (cells/1ml)	10.0 x 10^6
Viability at cell freezing (%)	96	Antibiotics Used	free
Passage Number	Unknown (P7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1075.0	Cell Name	IM95
LOT No.	09302016	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (4.5g/L glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) and 10 mg/L insulin.	Temperature	37 C
Cell Density at Seeding	0.7 - 1.6 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 35 hrs.	Cell Number in Vial (cells/1ml)	3.4 x 10^6
Viability at cell freezing (%)	76	Antibiotics Used	free
Passage Number	Unknown (7 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM (high glucose)	CO2 Conc.	5%
Viability immediately after thawing (%)	76	Additional information

Cell No.	JCRB1075.0	Cell Name	IM95
LOT No.	03302021	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (high glucose) with 10 mg/L insulin and 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.5 - 1.0 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 46 hrs	Cell Number in Vial (cells/1ml)	3.4 x 10^6
Viability at cell freezing (%)	80	Antibiotics Used	free
Passage Number	Unknown (6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - DMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	78	Additional information