JCRB0220 NTI-4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0220 Cell Name   NTI-4
Profile   Normal human fibroblast. Other Name   NTI-4
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   9-week fetus
Identity   available Tissue for Primary Cancer   embryo
Case history   normal Metastasis   
Tissue Metastasized    Genetics   fetus, half body, bottom/fibroblast
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   fetus, half body, bottom/fibroblast
Classify    Established by   Inui,N.
Registered by   Egashira,Y. Regulation for Distribution   
Comment    Year   1986
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25% trypsin.
Cell Number on Passage   2x10^4 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   fetus body
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3080
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LOT Information

Cell No.   JCRB0220 Cell Name   
LOT No.   01112008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS Lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 20 hrs Cell Number in Vial (cells/1ml)   7 x 10^5
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   P18 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0220 Cell Name   
LOT No.   042592 Lot Specification   distribution
Medium   DMDM : 10% FCS Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0220 Cell Name   NTI-4
LOT No.   04162024 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   4.4 - 9.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P19 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:8
D7S820:9,10
D16S539:9,12
VWA:16,19
TH01:7,8
AM:X
TPOX:8
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
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