JCRB0229 EGV-4T

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0229 Cell Name   EGV-4T
Profile   immortal cell line from MNNG-transformed tracheal epithelial cells Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   immortal cell line from MNNG-transformed cells
Life Span   infinite Crisis PDL   
Morphology    Character   rat tracheal epithelial cells
Classify   transformed Established by   Nettesheim,P.
Registered by   Egashira, Y. Regulation for Distribution   
Comment    Year   1987
Medium   Ham's F12 medium with 5-10% fetal calf serum Methods for Passages   Cells are treated with 0.05 % trypsin and 0.02 % EDTA.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   trachea
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5500
Reference
Pubmed id:4005856Changes in stem cell populations of rat tracheal epithelial cell cultures at an early stage in neoplastic progression.
Thomassen D,Barrett JC,Beeman DK,Nettesheim P
Cancer Res. 1985 Jul;45(7):3322-31

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LOT Information

Cell No.   JCRB0229 Cell Name   
LOT No.   03222005 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS lot; GIBCO4218333S) Temperature   37 C
Cell Density at Seeding   7 - 18 x 104 cells/ml Methods for Passages   0.05% trypsin and 0.02% EDTA for 10 min at 37 C
Doubling Time   approx. 37 hrs Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   P11 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0229 Cell Name   
LOT No.   092986 Lot Specification   distribution
Medium   F-12 + FCS 5-10% Temperature   37
Cell Density at Seeding   2x10^4/ml Methods for Passages   0.05 % trypsin, 0.02 % EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6.0 x 10^5
Viability at cell freezing (%)   89.6 Antibiotics Used   
Passage Number   unknown PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0229 Cell Name   EGV-4T
LOT No.   09092020 Lot Specification   distribution
Medium   Ham's F-12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   97.6 Additional information   
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