JCRB0233.0 BALB-MC

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0233.0 Cell Name   BALB-MC
Profile   mammary cancer, (BALB/cHe mouse) Other Name   BALB/c-MC
Animal   mouse Strain   BALB/cHe
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   mammary
Case history    Metastasis   
Tissue Metastasized    Genetics   mammary cancer
Life Span   infinite Crisis PDL   
Morphology    Character   mammary cancer
Classify    Established by   Morimoto,J.
Registered by   Egashira,Y. Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.12-0.24 % trypsin and 0.01-0.02 % EDTA.
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   mammary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J361
Reference
Pubmed id:3680103 Establishment and characterization of a new murine mammary tumor cell line, BALB/c-MC.
Morimoto J,Imai S,Taniguchi Y,Tsubura Y,Hosick HL
In Vitro Cell Dev Biol. 1987 Nov;23(11):755-8

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LOT Information

Cell No.   JCRB0233.0 Cell Name   
LOT No.   051187 Lot Specification   distribution
Medium   MEM + 10 % FCS Temperature   37
Cell Density at Seeding   1/4 - 1/5 Methods for Passages   0.12-0.24 % trypsin + 0.01-0.02 % EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   UNK
Viability at cell freezing (%)   NT Antibiotics Used   penicilline/streptomycin
Passage Number   *P3 PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD, G6PD, NP, PEPB, AST, MD, MPI
Chromosome Mode   45 Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0233.0 Cell Name   
LOT No.   111488 Lot Specification   distribution
Medium   MEM + 10 % FCS Temperature   37
Cell Density at Seeding   1/4 - 1/5 Methods for Passages   0.12-0.24 % trypsin + 0.01-0.02 % EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P17 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD, G6PD, NP, PEPB, AST, MD, MPI
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0233.0 Cell Name   BALB-MC
LOT No.   02162015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.4 - 1.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 - 33 hrs Cell Number in Vial (cells/1ml)   4.0 x 10^6
Viability at cell freezing (%)   65.2 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   65.2
Additional information   
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