JCRB1078 TMH-1

Cell information

Important Notice(s)

Cross contamination in JCRB1078 TMH-1 cells.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1078 Cell Name   TMH-1
Profile   Human adherent monolayer cell line with multidrug resistance obtained from a benign goiter and chronic thyroiditis. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   79
Identity   available Tissue for Primary Cancer   thyroid
Case history   benign goiter and chronic thyroiditis, radiotherapy at a total dose of 43.5 Gy without chemotherapy Metastasis   
Tissue Metastasized    Genetics   Choromosome No. ditributes from 40 to 120.
Life Span   infinite Crisis PDL   
Morphology   spindle-shaped with giant cells Character   Doubling time is 74 hours. Multidrug resistance.
Classify   tumor Established by   Sekiguchi,M. et.al.
Registered by   Sekiguchi,M. Regulation for Distribution   not applied
Comment   Cross contamination of IHH-4 cell line is suspected. Year   2003
Medium   1 to 1 mixture of Dulbecco's modified Eagle's medium and RPMI1640 medium containing 10% heat inactivated fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin.
Cell Number on Passage   10^4 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   thyroid(goiter)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3219
Pubmed id:11686581Biological characteristics and chemosensitivity profile of four human anaplastic thyroid carcinoma cell lines.
Sekiguchi M,Shiroko Y,Arai T,Kishino T,Sugawara I,Kusakabe T,Suzuki T,Yamashita T,Obara T,Ito K,Hasumi K
Biomed Pharmacother. 2001 Oct;55(8):466-74


LOT Information

Cell No.   JCRB1078 Cell Name   TMH-1
LOT No.   11272003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin. Subculture once a week. Medium change once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   99.0 Antibiotics Used   free
Passage Number   P304 PDL   42
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information