JCRB0234.0 TYK-nu

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0234.0 Cell Name   TYK-nu
Profile   undifferentiated carcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   ovary
Case history    Metastasis   
Tissue Metastasized    Genetics   ovary, undifferentiated carcinoma
Life Span    Crisis PDL   
Morphology    Character   ovary, undifferentiated carcinoma
Classify    Established by   Yoshiya,N.
Registered by   Egashira, Y. Regulation for Distribution   Make notice to the originator on distribution.
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25 % trypsin, 0.1-0.01 % EDTA (4/1)
Cell Number on Passage   2x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1776
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909774
Reference
Pubmed id:3782955[Establishment of a cell line from human ovarian cancer (undifferentiated carcinoma of FIGO classification) and analysis of its cell-biological characteristics and sensitivity to anticancer drugs].
Yoshiya N
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Oct;38(10):1747-53

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LOT Information

Cell No.   JCRB0234.0 Cell Name   
LOT No.   012099 Lot Specification   distribution
Medium   Eagle's minimal essential medium + 10% fetal calf serum (Biowhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 50hrs Cell Number in Vial (cells/1ml)   1.8 X10^6
Viability at cell freezing (%)   86% Antibiotics Used   free
Passage Number   P9 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0234.0 Cell Name   
LOT No.   100794 Lot Specification   distribution
Medium   MEM + 10 % FCS Temperature   37
Cell Density at Seeding   1/5 - 1/20 Methods for Passages   0.25% trypsin, 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P106 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST, G6PD, LD, MD, MPI, NP, PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0234.0 Cell Name   TYK-nu
LOT No.   07182013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FBS; GIBCO Cat. # 10099) Temperature   37C
Cell Density at Seeding   6.4 x 10^4 cells/mL Methods for Passages   Cells are treated with 0.25 % trypsin, 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.2 x 10^6 (cells/1
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   92.4 Additional information   
Cell No.   JCRB0234.0 Cell Name   TYK-nu
LOT No.   08192016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.0 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 44 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   83 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
Cell No.   JCRB0234.0 Cell Name   TYK-nu
LOT No.   02252019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.5 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
Cell No.   JCRB0234.0 Cell Name   TYK-nu
LOT No.   02132023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   0.4-1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   28 - 34 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:10,11
D7S820:10
D16S539:9,10
VWA:14,16
TH01:9
AM:X
TPOX:9,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95.1 Additional information   
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