JCRB0237 LMH

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0237 Cell Name   LMH
Profile   hepatocelluler carcinoma, diethylnitrosamine induced Other Name   
Animal   chicken Strain   
Genus    Species   
Sex    Age   
Identity    Tissue for Primary Cancer   liver
Case history    Metastasis   
Tissue Metastasized    Genetics   liver cancer induced by DEN
Life Span    Crisis PDL   
Morphology    Character   liver cancer induced by DEN
Classify    Established by   Cancer Inst.JPN
Registered by   Cancer Inst. JPN Regulation for Distribution   
Comment   Virus free cell line which have a potential activity for differentiation of a liver cell. Possibly used for studies of virus infection, replication, and etc. Year   1988
Medium   Waymouth's MB752/1 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.05 % trypsin and 0.02 % EDTA.
Cell Number on Passage   split ratio=1/4 Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2580
Reference
Pubmed id:3607775Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH.
Kawaguchi T,Nomura K,Hirayama Y,Kitagawa T
Cancer Res. 1987 Aug 15;47(16):4460-4

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LOT Information

Cell No.   JCRB0237 Cell Name   
LOT No.   09142000 Lot Specification   distribution
Medium   Waymouth's MB752/1 medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   1.3-2.3 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca 40 hrs Cell Number in Vial (cells/1ml)   6.5 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0237 Cell Name   
LOT No.   042490 Lot Specification   distribution
Medium   Waymouth's MB752/1 + FCS 10% Temperature   37
Cell Density at Seeding    Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0237 Cell Name   LMH
LOT No.   10022014 Lot Specification   distribution
Medium   Waymouth's MB752/1 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.8 - 2.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02 % EDTA.
Doubling Time   approx. 50 hrs Cell Number in Vial (cells/1ml)   7.6 x 10^6
Viability at cell freezing (%)   85.1 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as chicken by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Waymouth's MB752/1 medium CO2 Conc.   5%
Viability immediately after thawing (%)   85.1 Additional information   
Cell No.   JCRB0237 Cell Name   LMH
LOT No.   06282019 Lot Specification   distribution
Medium   Waymouth's MB752/1 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012 Temperature   37 C
Cell Density at Seeding   1.0 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 day Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   Unknown (4 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Waymouth's MC752/1 medium CO2 Conc.   5%
Viability immediately after thawing (%)   74.3 Additional information   
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