JCRB0542 TIG-120

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0542 Cell Name   TIG-120
Profile   Normal diploid fibroblast from human skin with limited lifespan. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   6-years-old
Identity   available Tissue for Primary Cancer   skin
Case history   normal Metastasis   
Tissue Metastasized    Genetics   normal diploid (2n=46,XX)
Life Span   finite(PD40) Crisis PDL   PD40
Morphology   fibroblast-like Character   normal diploid fibroblast
Classify   normal Established by   TMIG
Registered by   TMIG Regulation for Distribution   NT
Comment   NT Year   2004
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   *
Cell Number on Passage   split ratio = 1/4 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   normal diploid fibroblast

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3202
Reference
Pubmed id:7556460 Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.
Kondo H,Yonezawa Y
Exp Cell Res. 1995 Oct;220(2):501-4

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LOT Information

Cell No.   JCRB0542 Cell Name   TIG-120
LOT No.   07122011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (This lot was frozen using DMSO.) Temperature   37 C
Cell Density at Seeding   approx. 3.2 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   6.3 x 10^5
Viability at cell freezing (%)   93.7 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   21
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0542 Cell Name   
LOT No.   08202010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2.6 - 3.9 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 45 hrs Cell Number in Vial (cells/1ml)   5.2 x 10^5
Viability at cell freezing (%)   90.5 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   21
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0542 Cell Name   TIG-120
LOT No.   04152013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS; GIBCO Cat # 10099). This lot was frozen using DMSO. Label of vial was mistyped. Correct JCRB# is JCRB0542. Temperature   37 C
Cell Density at Seeding   2.9 - 4.3 x 10^4 cells/mL, split ratio = 1/4 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Doubling time: 26 - 48 hrs.
Doubling Time    Cell Number in Vial (cells/1ml)   8.4 x 10^5
Viability at cell freezing (%)   97.7 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   21.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   97.7
Additional information   The vials are labeled as JCRB1542 in error. The correct number is JCRB0542.
Cell No.   JCRB0542 Cell Name   TIG-120
LOT No.   09112018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   3.5 - 8.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   7.2 x 10^5
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   21.3
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FSB - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.7
Additional information   
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