JCRB1080.0 HARA

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1080.0 Cell Name   HARA
Profile   Human lung squamous cell carcinoma with PTHrP expression. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   57
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   This cell line expresses PTHrP highly and the bone metastasis is induced extensively by the injection to the heart chamber of nude mouse. Hypercalcemia is induced in mice by hypodermic transplantation of the cell.
Classify   tumor Established by   Iguchi,H.
Registered by   Iguchi,H. Regulation for Distribution   
Comment   STR analysis indicated that the cell line is unique (06/02/2004). Year   2004
Medium   RPMI1640 medium with 10% fetal bovine serum. (Antibiotics). Methods for Passages   medium change every 3 days.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   pleural fluid
Tissue Type   human squamous cell lung carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2914
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240142
Reference
Pubmed id:11668474Involvement of parathyroid hormone-related protein in experimental cachexia induced by a human lung cancer-derived cell line established from a bone metastasis specimen.
Iguchi H,Onuma E,Sato K,Sato K,Ogata E
Int J Cancer. 2001 Oct 1;94(1):24-7
Pubmed id:8752176An experimental model of bone metastasis by human lung cancer cells: the role of parathyroid hormone-related protein in bone metastasis.
Iguchi H,Tanaka S,Ozawa Y,Kashiwakuma T,Kimura T,Hiraga T,Ozawa H,Kono A
Cancer Res. 1996 Sep 1;56(17):4040-3
Pubmed id:8287365Establishment of lung cancer cell line producing parathyroid hormone-related protein.
Ichinose Y,Iguchi H,Ohta M,Katakami H
Cancer Lett. 1993 Oct 15;74(1-2):119-24
Pubmed id:8514608A case of squamous cell lung carcinoma with high concentration of parathyroid hormone-related peptide in serum and pleural effusion presenting hypercalcemia.
Iguchi H,Katakami H,Ichinose Y,Nishi Y,Tanaka S,Hara N,Ohta M,Haji M,Nawata H
Jpn J Cancer Res. 1993 Apr;84(4):419-24

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LOT Information

Cell No.   JCRB1080.0 Cell Name   
LOT No.   12172007 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (lot:GIBCO484375) Temperature   37C
Cell Density at Seeding   1.5 x 10^5cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   approx. 30hr Cell Number in Vial (cells/1ml)   5 x 10^6 cells
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P8＊ PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B) MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1080.0 Cell Name   HARA
LOT No.   07072004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.01% EDTA. Subculture every 1-2 days and medium change every 3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.3 x 10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:9
D7S820:12
D16S539:10
VWA:16,17
TH01:7
AM:XY
TPOX:8,9
CSF1PO:13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cultuer medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1080.0 Cell Name   HARA
LOT No.   04142014 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.0 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   5.9 x 10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:9
D7S820:12
D16S539:10
VWA:16,17
TH01:7
AM:X,Y
TPOX:8,9
CSF1PO:13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   98.0 Additional information   
Cell No.   JCRB1080.0 Cell Name   HARA
LOT No.   05242021 Lot Specification   distribution
Medium   "RPMI1640 medium(GIBCO：Cat No.;11875-093) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012-500ML,Lot#12J396) " Temperature   37 C
Cell Density at Seeding   1.5-3.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.98x10^6
Viability at cell freezing (%)   91.80 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:9
D7S820:12
D16S539:10
VWA:16,17
TH01:7
AM:X,Y
TPOX:8,9
CSF1PO:13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94.7 Additional information   
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