JCRB0240 MCAS

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0240 Cell Name   MCAS
Profile   mucinous cystadenocarcinoma Other Name   MCAS
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   ovary
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology    Character   
Classify    Established by   Kidera,Y.
Registered by   Iwasaka,T. Regulation for Distribution   
Comment    Year   1988
Medium   Eagle's minimal essential medium with 20% fetal calf serum Methods for Passages   0.025 % EDTA-0.02 % trypsin, 3-5 min at 37 C. Split ratio = 1/5
Cell Number on Passage   5.4x10^4 cells/cm^2 Race   
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3020
Reference
Pubmed id:4056530[Establishment and characterization of a cell line derived from mucinous cystadenocarcinoma of human ovary].
Kidera Y,Yoshimura T,Ohkuma Y,Iwasaka T,Sugimori H
Nihon Sanka Fujinka Gakkai Zasshi. 1985 Sep;37(9):1820-4

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LOT Information

Cell No.   JCRB0240 Cell Name   MCAS
LOT No.   01202012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 20% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.5 - 3.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Pssage twice a week.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90%
Additional information   
Cell No.   JCRB0240 Cell Name   
LOT No.   03262008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 20% fetal bovine serum (GIBCO Cat.#10099, lot 484375) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA at 37 C for 10 min.
Doubling Time   approx. 28 hrs Cell Number in Vial (cells/1ml)   6.7 x 10^5
Viability at cell freezing (%)   91.5 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0240 Cell Name   MCAS
LOT No.   062395 Lot Specification   distribution
Medium   Eagle's minimal essential medium with fetal bovine serum (20%) (JRH12103-78P) Temperature   37 C
Cell Density at Seeding   2.1x10^4 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.5 x 10^5
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   P20 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0240 Cell Name   MCAS
LOT No.   08162016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.5 - 1.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   87.1 Antibiotics Used   free
Passage Number   Unknown (22 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   87.1
Additional information   
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