JCRB0263.1 PC12HS.P3

Cell information

Important Notice(s)

無血清馴化株PC12.P3, PC12HS.P3について

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0263.1 Cell Name   PC12HS.P3
Profile   Serum free NGF sensitive PC12HS cell line. Other Name   PC12 clone HS.P3
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   pheochromocytoma of the adrenal medulla. Metastasis   No
Tissue Metastasized    Genetics   Serum free cell culture derived from NGF-highly sensitive clone (HS)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Cells are differentiated into sympathetic neuron-like extensions when they are exposed to NGF.
Classify   variant Established by   Takaoka,T.
Registered by   Takaoka,T. Regulation for Distribution   
Comment    Year   2004
Medium   DM201. Serum free. Methods for Passages   Cells were harvested with scraper.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   chromaffin cell
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J439
Pubmed id:1493541Different ability in PC12 clones to recover from MMC toxicity following NGF treatment.
Iizuka H,Umeda M
Brain Res. 1992 Dec 18;599(1):1-5
Pubmed id:3839317Molecular cloning of a gene sequence regulated by nerve growth factor.
Levi A,Eldridge JD,Paterson BM
Science. 1985 Jul 26;229(4711):393-5
Pubmed id:6641712A macromolecular structure favouring microtubule assembly in NGF-differentiated pheochromocytoma cells (PC12).
Biocca S,Cattaneo A,Calissano P
EMBO J. 1983;2(5):643-8
Pubmed id:742018[Therapeutic results in adult acute leukemia in Giessen].
Löffler H,Pralle H,Graubner M,Schrezenmaier E
Verh Dtsch Ges Inn Med. 1978;(84):156-8
Pubmed id:1065897Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Greene LA,Tischler AS
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8


LOT Information

Cell No.   JCRB0263.1 Cell Name   PC12HS.P3
LOT No.   08042004 Lot Specification   distribution
Medium   DM201 medium. Serum free medium. Temperature   37 C
Cell Density at Seeding   1-3 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested with scraper. Subculture every 4-6 days and medium change every 2-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.3 x 10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   MC210 to remove mycoplasmas. This culture only.
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   1 to 1 mixture of the culture medium with the FM-1 medium containing 10% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information