JCRB0718.1 RLC-10.P3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0718.1 | Cell Name | RLC-10.P3 |
|---|---|---|---|
| Profile | Rat normal cell line cultured with serum free medium for long passages. | Other Name | |
| Animal | rat | Strain | JAR-1 |
| Genus | Rattus | Species | norvegicus |
| Sex | F | Age | 11-day-old |
| Identity | Tissue for Primary Cancer | liver | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | Telomelase was negative and a length of telomere was 2.0 Kbases. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | |
| Classify | normal | Established by | Takaoka,T. |
| Registered by | Takaoka,T. | Regulation for Distribution | free |
| Comment | Year | 2004 | |
| Medium | Serum free DM201 medium. | Methods for Passages | Cells were harvested by scraper gently and suspended in fresh medium. |
| Cell Number on Passage | 1x10^6 cells/ml | Race | |
| CO2 Conc. | Tissue Sampling | liver | |
| Tissue Type | normal |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:4358144 | Toxic metabolites released from rat hepatoma cells in culture. I. Effects of metabolites of hepatomas on various cells. Katsuta H,Takaoka T,Yasumoto S J Natl Cancer Inst. 1973 Dec;51(6):1841-4 |
| Pubmed id:4647843 | Carcinogenesis in tissue culture. XIV. Malignant transformation of rat liver parenchymal cells treated with 4-nitroquinoline 1-oxide in tissue culture. Katsuta H,Takaoka T J Natl Cancer Inst. 1972 Dec;49(6):1563-76 |
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LOT Information
| Cell No. | JCRB0718.1 | Cell Name | RLC-10.P3 |
|---|---|---|---|
| LOT No. | 09222004 | Lot Specification | distribution |
| Medium | DM201 medium. Serum free. | Temperature | 37 C |
| Cell Density at Seeding | 1.1 x 10^4 cells/sq.cm. | Methods for Passages | Cells were harvested with scraper, subculture 7-10 days. Medium change every 2-3 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.4 x 10^6 |
| Viability at cell freezing (%) | 78.6 | Antibiotics Used | free |
| Passage Number | P9* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | 1 to 1 mixture of the culture medium and the FM-1 medium containing final 10% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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